Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3869274..3869928 Replicon chromosome
Accession NZ_CP058618
Organism Escherichia coli strain JS316

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZF20_RS18645 Protein ID WP_000244777.1
Coordinates 3869521..3869928 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZF20_RS18640 Protein ID WP_000354046.1
Coordinates 3869274..3869540 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZF20_RS18615 3864562..3865305 + 744 Protein_3624 SDR family oxidoreductase -
HZF20_RS18620 3865362..3866795 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HZF20_RS18625 3866840..3867151 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
HZF20_RS18630 3867315..3867974 + 660 WP_000250274.1 hemolysin III family protein -
HZF20_RS18635 3868051..3869031 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZF20_RS18640 3869274..3869540 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZF20_RS18645 3869521..3869928 + 408 WP_000244777.1 toxin CptA Toxin
HZF20_RS18650 3869968..3870489 - 522 WP_001055874.1 flavodoxin FldB -
HZF20_RS18655 3870601..3871497 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZF20_RS18660 3871522..3872232 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZF20_RS18665 3872238..3873971 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T166954 WP_000244777.1 NZ_CP058618:3869521-3869928 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T166954 NZ_CP074572:2963923-2964177 [Shewanella dokdonensis]
ATGAGAATCGTATCTTTTACTGAAGCTCGTAATAGTTTGAAAAGTGTCTTAGATGGTGTGGTTAATGATGTCGACACAAC
AGTTATTACCCGCCGAGATGCTGAAGATGCTGTAGTTATGTCCTTAGATTATTACAACAGCCTAATGGAAACTGTTCATT
TGTTGCGTTCTCCAGCCAATGCCGAGCATTTGAATCGTTCAATTGCCCAATACAAAGCTGGTCAAACAACACTACGGGAC
TTGATTGATGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT166954 WP_000354046.1 NZ_CP058618:3869274-3869540 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT166954 NZ_CP074572:2964170-2964433 [Shewanella dokdonensis]
ATGAATAGTCGTTTACTGTCCTGGACAGATGAAGCTTGGAACGACTATGAGTATTGGCAACCACAAGATAAGAAAACCTT
AAAACGGATCAATAAATTAATCACAAATGTTAAACGTGCTCCTTTTGAGGGTATCGGTAAACCGGAGGCTCTAAAGGAGA
ACTTAGCTGGTTTTTGGTCACGGCGAATAGACGATAAAAATCGCTTAGTTTACGCCGTAGACGATCAAGCAATAACAATC
CTGTCATGTCGTTATCATTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References