Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1722405..1723023 Replicon chromosome
Accession NZ_CP058618
Organism Escherichia coli strain JS316

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HZF20_RS08370 Protein ID WP_001291435.1
Coordinates 1722805..1723023 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HZF20_RS08365 Protein ID WP_000344800.1
Coordinates 1722405..1722779 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZF20_RS08355 1717494..1718687 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HZF20_RS08360 1718710..1721859 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HZF20_RS08365 1722405..1722779 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HZF20_RS08370 1722805..1723023 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HZF20_RS08375 1723195..1723746 + 552 WP_000102568.1 maltose O-acetyltransferase -
HZF20_RS08380 1723862..1724332 + 471 WP_000136192.1 YlaC family protein -
HZF20_RS08385 1724496..1726046 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HZF20_RS08390 1726088..1726441 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HZF20_RS08400 1726820..1727131 + 312 WP_000409911.1 MGMT family protein -
HZF20_RS08405 1727162..1727734 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166947 WP_001291435.1 NZ_CP058618:1722805-1723023 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166947 NZ_CP074570:3381297-3381590 [Paraneptunicella aestuarii]
GTGACAACTTATTTACTTTCTCCCGCAGCTCAAGCAAGCCTGAAAAACATCAAAGCCTATTCCCTCGAAAACTTTGGAAC
AATACAAACTCGACATTACCTGAAAAGCTTAAGAGAAAGAATGCAGGCACTGGCAGAAAACCCTGAACGAGGGCGAATTC
GCGATGAATTGAAGGTGGGATTTTATAGCGACTTCATAGGCTCACACACTATTTATTACCGAATCAAACCGCCCCATATC
GAAATCATTGATGTACTGCATCAATCAATGGATCCCAGCAAACACGTAGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166947 WP_000344800.1 NZ_CP058618:1722405-1722779 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166947 NZ_CP074570:3381061-3381297 [Paraneptunicella aestuarii]
ATGCAGAGAAATACCAGCGTGACATTGGGCGAGCACTTTGGGCAATTCATTGAACAAAAAGTGCAACAAGGCCGATTTGA
ATCAACCAGTGAAGCCGTTCGAGCTGGTCTTCGATTACTTGAAGAGCAAGAAATGAAACTGGATATACTGAGAAAAAAAC
TTGAAGTCGGTGAAACACAGTTGGATCGAGGCGAAGGTATTGATGGTGATAAATTTATGCAGGATCTGATTGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References