Detailed information of TA system TA166946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 622157..623745
• Replicon: chromosome
• Accession: NZ_CP058618
• Organism: Escherichia coli strain JS316

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: HZF20_RS03005
• Protein ID: WP_001125463.1
• Coordinates: 622423..623745 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HZF20_RS03000
• Protein ID: WP_001296726.1
• Coordinates: 622157..622423 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZF20_RS03000	622157..622423	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HZF20_RS03005	622423..623745	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HZF20_RS03010	624057..624265	+	209	Protein_587	helix-turn-helix transcriptional regulator	-
HZF20_RS03015	624697..625260	+	564	WP_001373493.1	type 1 fimbrial major subunit FimA	-
HZF20_RS03020	625621..626331	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	622157..639581	17424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T166946 WP_001125463.1 NZ_CP058618:622423-623745 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T166946 NZ_CP074570:3010032-3010304 [Paraneptunicella aestuarii]
ATGCTTAATTTAGAGTATTCATCACAATTCAAGAAAGACTTTAAGAAGATCACCAAATTGCCTATTGCTGATATTGTCGA
AGTTGGTCATGTCATTAAGCAATTGCAGCTTGGCAACCCATTAGCAGAAAAATATGTTGATCATGCATTATCAGGAAATT
GGCAGCACTACCGCGACTGCCATATCAAACCAGATTTAGTGCTAATTTACAAAGCGGAAACAAGCACTTTAAAGCTAGCT
AGAATAGGCTCCCACAGCGAATTATTCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT166946 WP_001296726.1 NZ_CP058618:622157-622423 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT166946 NZ_CP074570:3009761-3010039 [Paraneptunicella aestuarii]
ATGAAAACAGAAATGCTAAGCACGCGGATAGATCCGGAAACCAAAATAGCATTTACCAATGTCTGCGAAGAAATGGGCCT
AAGCCCTTCGCAAGCGATTAAACTTTTTGCAAAAGCCGTGATTAATTATGGTGGCATCCCTTTTGAACTTAAAGCAAAAC
AGCCCAATGCCATAACAGCATCGGCGATCCAGGAACTTGAATCTGGCAAAGGCAACAAAGCAAAAGATGTAGATGAACTA
TTTGAAACCATGGGTATTAGTCAGGGACGCGATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0