Detailed information of TA system TA166916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 779957..780486
• Replicon: chromosome
• Accession: NZ_CP058613
• Organism: Staphylococcus aureus strain 128254

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HZR13_RS03860
• Protein ID: WP_000621175.1
• Coordinates: 780124..780486 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HZR13_RS03855
• Protein ID: WP_000948331.1
• Coordinates: 779957..780127 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR13_RS03825	774993..775553	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
HZR13_RS03830	775762..776241	+	480	WP_001287088.1	hypothetical protein	-
HZR13_RS03835	776234..777817	+	1584	WP_001294626.1	PH domain-containing protein	-
HZR13_RS03840	777804..778295	+	492	WP_001205910.1	PH domain-containing protein	-
HZR13_RS03845	778299..778658	+	360	WP_000581200.1	holo-ACP synthase	-
HZR13_RS03850	778724..779872	+	1149	WP_001281145.1	alanine racemase	-
HZR13_RS03855	779957..780127	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HZR13_RS03860	780124..780486	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZR13_RS03865	780836..781837	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HZR13_RS03870	781956..782282	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HZR13_RS03875	782284..782763	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
HZR13_RS03880	782738..783508	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T166916 WP_000621175.1 NZ_CP058613:780124-780486 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T166916 NZ_CP074553:736779-737264 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT166916 WP_000948331.1 NZ_CP058613:779957-780127 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT166916 NZ_CP074553:736489-736782 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1