Detailed information of TA system TA166893

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3038410..3039211
• Replicon: chromosome
• Accession: NZ_CP058596
• Organism: Escherichia coli strain UPEC_U013

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: HYE75_RS14520
• Protein ID: WP_000854739.1
• Coordinates: 3038410..3038790 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: HYE75_RS14525
• Protein ID: WP_001285482.1
• Coordinates: 3038837..3039211 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYE75_RS14485	3034001..3034555	-	555	WP_001001909.1	molecular chaperone YcdY	-
HYE75_RS14490	3034579..3035316	-	738	WP_000283664.1	zinc-binding phosphatase	-
HYE75_RS14495	3035371..3036309	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HYE75_RS14505	3036796..3037623	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
HYE75_RS14510	3037708..3037905	-	198	WP_000772027.1	DUF957 domain-containing protein	-
HYE75_RS14515	3037925..3038413	-	489	WP_001054232.1	hypothetical protein	-
HYE75_RS14520	3038410..3038790	-	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
HYE75_RS14525	3038837..3039211	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HYE75_RS14530	3039261..3039905	-	645	WP_000086763.1	hypothetical protein	-
HYE75_RS14535	3039924..3040145	-	222	WP_000692315.1	DUF987 domain-containing protein	-
HYE75_RS14540	3040208..3040684	-	477	WP_001186170.1	RadC family protein	-
HYE75_RS14545	3040700..3041173	-	474	WP_001537423.1	antirestriction protein	-
HYE75_RS14550	3041436..3042257	-	822	WP_097424173.1	DUF945 domain-containing protein	-
HYE75_RS14555	3042338..3042549	+	212	Protein_2858	hypothetical protein	-
HYE75_RS14560	3042668..3043123	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T166893 WP_000854739.1 NZ_CP058596:c3038790-3038410 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T166893 NZ_CP074541:324617-324985 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT166893 WP_001285482.1 NZ_CP058596:c3039211-3038837 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT166893 NZ_CP074541:324399-324620 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H2V687

Antitoxin

• AlphaFold DB: A0A0P7R0L9