Detailed information of TA system TA166876

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 45040..45838
• Replicon: chromosome
• Accession: NZ_CP058596
• Organism: Escherichia coli strain UPEC_U013

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HYE75_RS00225
• Protein ID: WP_016238198.1
• Coordinates: 45040..45417 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: HYE75_RS00230
• Protein ID: WP_001285415.1
• Coordinates: 45464..45838 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYE75_RS00190	40174..40467	-	294	WP_001297978.1	hypothetical protein	-
HYE75_RS00195	40689..41507	+	819	WP_000779409.1	lipoprotein NlpA	-
HYE75_RS00200	41511..42434	-	924	WP_001351210.1	carboxylate/amino acid/amine transporter	-
HYE75_RS00205	42601..43098	-	498	WP_000509808.1	hypothetical protein	-
HYE75_RS00210	43694..44536	-	843	WP_001521669.1	DUF4942 domain-containing protein	-
HYE75_RS00215	44621..44818	-	198	WP_086795284.1	DUF957 domain-containing protein	-
HYE75_RS00220	44894..45043	-	150	Protein_43	hypothetical protein	-
HYE75_RS00225	45040..45417	-	378	WP_016238198.1	TA system toxin CbtA family protein	Toxin
HYE75_RS00230	45464..45838	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HYE75_RS00235	45918..46139	-	222	WP_000692328.1	DUF987 domain-containing protein	-
HYE75_RS00240	46208..46684	-	477	WP_016238197.1	RadC family protein	-
HYE75_RS00245	46700..47185	-	486	WP_016238196.1	antirestriction protein	-
HYE75_RS00250	47277..48095	-	819	WP_021546487.1	DUF945 domain-containing protein	-
HYE75_RS00255	48185..48394	-	210	WP_032150870.1	DUF905 family protein	-
HYE75_RS00260	48424..49101	-	678	WP_021546486.1	hypothetical protein	-
HYE75_RS00265	49220..50104	-	885	WP_021546485.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14206.16 Da
• Isoelectric Point: 6.9576

>T166876 WP_016238198.1 NZ_CP058596:c45417-45040 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T166876 NZ_CP074535:c5029882-5029598 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT166876 WP_001285415.1 NZ_CP058596:c45838-45464 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT166876 NZ_CP074535:c5030114-5029872 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A067H947