Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3284108..3284741 Replicon chromosome
Accession NZ_CP058594
Organism Weizmannia coagulans strain ASRS217

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag HYE74_RS15645 Protein ID WP_013860680.1
Coordinates 3284108..3284458 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag HYE74_RS15650 Protein ID WP_014096808.1
Coordinates 3284463..3284741 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYE74_RS15610 3279364..3280143 - 780 WP_014096815.1 RNA polymerase sigma factor SigB -
HYE74_RS15615 3280121..3280591 - 471 WP_014096814.1 anti-sigma B factor RsbW -
HYE74_RS15620 3280595..3280930 - 336 WP_026104587.1 anti-sigma factor antagonist -
HYE74_RS15625 3281000..3282010 - 1011 WP_017551130.1 PP2C family protein-serine/threonine phosphatase -
HYE74_RS15630 3282024..3282425 - 402 WP_014096811.1 anti-sigma regulatory factor -
HYE74_RS15635 3282430..3282786 - 357 WP_014096810.1 STAS domain-containing protein -
HYE74_RS15640 3282790..3283617 - 828 WP_014096809.1 RsbT co-antagonist protein RsbRA -
HYE74_RS15645 3284108..3284458 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HYE74_RS15650 3284463..3284741 - 279 WP_014096808.1 hypothetical protein Antitoxin
HYE74_RS15655 3284894..3286045 - 1152 WP_014096807.1 alanine racemase -
HYE74_RS15660 3286247..3287263 - 1017 WP_014096806.1 outer membrane lipoprotein carrier protein LolA -
HYE74_RS15665 3287404..3287754 - 351 WP_014096805.1 holo-ACP synthase -
HYE74_RS15670 3288035..3288634 + 600 WP_014096804.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T166875 WP_013860680.1 NZ_CP058594:c3284458-3284108 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T166875 NZ_CP074535:c4928038-4927505 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT166875 WP_014096808.1 NZ_CP058594:c3284741-3284463 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT166875 NZ_CP074535:c4928315-4928049 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References