Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54330..54855 Replicon plasmid pEC1-1
Accession NZ_CP058575
Organism Escherichia coli strain EC1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HYH05_RS24805 Protein ID WP_001159871.1
Coordinates 54550..54855 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HYH05_RS24800 Protein ID WP_000813630.1
Coordinates 54330..54548 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH05_RS24785 49745..50065 + 321 WP_001363097.1 hypothetical protein -
HYH05_RS24790 50156..51501 - 1346 Protein_61 IS4-like element IS4 family transposase -
HYH05_RS24795 51568..52011 - 444 Protein_62 hypothetical protein -
HYH05_RS24800 54330..54548 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HYH05_RS24805 54550..54855 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HYH05_RS24810 54856..55662 + 807 WP_000016970.1 site-specific integrase -
HYH05_RS24815 56383..57138 + 756 WP_000852146.1 replication initiation protein RepE -
HYH05_RS24820 57726..58892 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) - 1..122379 122379


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T166864 WP_001159871.1 NZ_CP058575:54550-54855 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T166864 NZ_CP074529:41208-41714 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCACCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTTCTCGA
TGAATGGTTAAGACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACCG
AAGAAGCTAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTATTCCTTAAGCTTCCA
AGACTAATTATTGGTGTGGGAAAATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT166864 WP_000813630.1 NZ_CP058575:54330-54548 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT166864 NZ_CP074529:40954-41220 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TCAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References