Detailed information of TA system TA166853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3247285..3247990
• Replicon: chromosome
• Accession: NZ_CP058574
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HYH05_RS15805
• Protein ID: WP_000539521.1
• Coordinates: 3247285..3247671 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HYH05_RS15810
• Protein ID: WP_001280945.1
• Coordinates: 3247661..3247990 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS15785	3243289..3243915	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HYH05_RS15790	3243912..3245027	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
HYH05_RS15795	3245138..3245521	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HYH05_RS15800	3245734..3247059	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HYH05_RS15805	3247285..3247671	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HYH05_RS15810	3247661..3247990	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HYH05_RS15815	3248060..3249388	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
HYH05_RS15820	3249396..3251743	-	2348	Protein_3105	CHASE9 sensor domain-containing protein	-
HYH05_RS15825	3251921..3252832	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T166853 WP_000539521.1 NZ_CP058574:3247285-3247671 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T166853 NZ_CP074528:691096-691437 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT166853 WP_001280945.1 NZ_CP058574:3247661-3247990 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT166853 NZ_CP074528:690758-691075 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6