Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 908132..908786 Replicon chromosome
Accession NZ_CP058574
Organism Escherichia coli strain EC1

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag HYH05_RS04420 Protein ID WP_000244769.1
Coordinates 908379..908786 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HYH05_RS04415 Protein ID WP_000354046.1
Coordinates 908132..908398 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH05_RS04395 904220..905653 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
HYH05_RS04400 905698..906009 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HYH05_RS04405 906173..906832 + 660 WP_000250274.1 hemolysin III family protein -
HYH05_RS04410 906909..907889 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
HYH05_RS04415 908132..908398 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HYH05_RS04420 908379..908786 + 408 WP_000244769.1 protein YgfX Toxin
HYH05_RS04425 908826..909347 - 522 WP_001055874.1 flavodoxin FldB -
HYH05_RS04430 909459..910355 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
HYH05_RS04435 910380..911090 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HYH05_RS04440 911096..912829 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T166839 WP_000244769.1 NZ_CP058574:908379-908786 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T166839 NZ_CP074523:324617-324985 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT166839 WP_000354046.1 NZ_CP058574:908132-908398 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT166839 NZ_CP074523:324399-324620 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References