Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 57267..57814 Replicon plasmid pEC2-1
Accession NZ_CP058572
Organism Escherichia coli strain EC2

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag HX136_RS25380 Protein ID WP_000970007.1
Coordinates 57267..57548 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HX136_RS25385 Protein ID WP_000079941.1
Coordinates 57545..57814 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HX136_RS25355 53903..54505 + 603 WP_000517694.1 hypothetical protein -
HX136_RS25360 54561..55055 + 495 WP_001459505.1 hypothetical protein -
HX136_RS25365 55055..55321 + 267 WP_000955263.1 hypothetical protein -
HX136_RS25370 55789..56262 - 474 WP_001334658.1 hypothetical protein -
HX136_RS25375 56379..57227 - 849 WP_021265674.1 3'-5' exonuclease -
HX136_RS25380 57267..57548 - 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HX136_RS25385 57545..57814 - 270 WP_000079941.1 hypothetical protein Antitoxin
HX136_RS25390 58181..59317 + 1137 WP_001250345.1 IS91 family transposase -
HX136_RS25395 59348..59581 + 234 WP_072042932.1 hypothetical protein -
HX136_RS25400 59782..60624 + 843 WP_001456218.1 alpha/beta fold putative hydrolase EstX -
HX136_RS25405 60713..61504 + 792 WP_001206316.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
HX136_RS25410 61674..62006 + 333 WP_000800531.1 quaternary ammonium compound efflux SMR transporter QacL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / sul3 / fosA3 / blaTEM-1B - 1..109494 109494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T166833 WP_000970007.1 NZ_CP058572:c57548-57267 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T166833 NZ_CP074519:c4603175-4602891 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT166833 WP_000079941.1 NZ_CP058572:c57814-57545 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT166833 NZ_CP074519:c4603407-4603165 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References