Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3891230..3891848 Replicon chromosome
Accession NZ_CP058571
Organism Escherichia coli strain EC2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HX136_RS19180 Protein ID WP_001291435.1
Coordinates 3891630..3891848 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HX136_RS19175 Protein ID WP_000344800.1
Coordinates 3891230..3891604 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HX136_RS19165 3886319..3887512 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HX136_RS19170 3887535..3890684 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HX136_RS19175 3891230..3891604 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HX136_RS19180 3891630..3891848 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HX136_RS19185 3892020..3892571 + 552 WP_000102564.1 maltose O-acetyltransferase -
HX136_RS19190 3892687..3893157 + 471 WP_032270236.1 YlaC family protein -
HX136_RS19195 3893321..3894871 + 1551 WP_179231538.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HX136_RS19200 3894913..3895266 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HX136_RS19210 3895645..3895956 + 312 WP_000409911.1 MGMT family protein -
HX136_RS19215 3895987..3896559 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166825 WP_001291435.1 NZ_CP058571:3891630-3891848 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166825 NZ_CP074449:c4959987-4959610 [Klebsiella michiganensis]
ATGCAAACACAACCTGTTCCCCCGACACAGGAGGCTTCACCACGCCCGTCACCAGTGGAGATCTGGCTCCGCCTTTTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGTTAAACGACACGCCATTTTGCAATGATGGTGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGCCCTGATACGCACCGATCGTCACGGTTTC
AGCGCAGAAACACAGTCCCCACTCATCAGCAGTATTGATATCCTTCGCGCCCGTAAGGCCTGTGGACTGATGGCACGCAA
TGGCTACAGACTGGTGACCGATATCACCACCGGCAAATACAGCGAGGTGACTCGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166825 WP_000344800.1 NZ_CP058571:3891230-3891604 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166825 NZ_CP074449:c4960396-4960037 [Klebsiella michiganensis]
TTGTCGAATAAAATACCGCCTGTAAACCACAATATCGCCGAACCCTGGTGGGGGCTTAAACGCGATATCACACCCTGTTT
TGGCGCTCGCCTGGTGCAGGAGGGCAACCGGCTGCACTATCTCGCCGACCGCGCCAGTATCACCGGGCAGTTCAGCGACG
CGGATTTACGCCATCTGGACCAGGCATTTCCGCTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAAT
CCCCGCCATCAGCACGGCGTCACGCTCTACGCAAAAGGGCTGACCTGCAACGCGGATACACTCGGCAGTTGCGGATATGT
CTATATAGCGATTTACCCGACACCTGCAACAACAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References