Detailed information of TA system TA166800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4212998..4213632
• Replicon: chromosome
• Accession: NZ_CP058556
• Organism: Streptomyces sp. CB04723

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HXS80_RS18125
• Protein ID: WP_179182355.1
• Coordinates: 4212998..4213420 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HXS80_RS18130
• Protein ID: WP_179182356.1
• Coordinates: 4213417..4213632 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS80_RS18100	4208684..4209310	+	627	WP_179182350.1	Crp/Fnr family transcriptional regulator	-
HXS80_RS18105	4209517..4210281	+	765	WP_179182351.1	hypothetical protein	-
HXS80_RS18110	4210652..4211386	+	735	WP_179182352.1	hypothetical protein	-
HXS80_RS18115	4211376..4211681	+	306	WP_179182353.1	helix-turn-helix domain-containing protein	-
HXS80_RS18120	4211678..4212865	+	1188	WP_179182354.1	hypothetical protein	-
HXS80_RS18125	4212998..4213420	-	423	WP_179182355.1	PIN domain nuclease	Toxin
HXS80_RS18130	4213417..4213632	-	216	WP_179182356.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HXS80_RS18135	4214018..4216378	+	2361	WP_179182357.1	type I restriction-modification system subunit M/S	-
HXS80_RS18140	4216375..4216575	+	201	WP_179182358.1	hypothetical protein	-
HXS80_RS18145	4216951..4217381	-	431	Protein_3596	hypothetical protein	-
HXS80_RS18150	4217378..4218283	-	906	WP_179182359.1	HNH endonuclease	-
HXS80_RS18155	4218340..4218456	+	117	Protein_3598	pyridine nucleotide-disulfide oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4206290..4230891	24601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-49)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15258.18 Da
• Isoelectric Point: 4.8887

>T166800 WP_179182355.1 NZ_CP058556:c4213420-4212998 [Streptomyces sp. CB04723]
VSAALFLIDTSALARFLRGGAEQFGWDQAASAGLIATCPVTELEFFYSARSAVDRARGIEDLKSLFGWVPIDDRAHDRAW
QVQELLTQRGQHRSAGAVDLIVAATAELQGLTLLHRDHDFECIAAVTGQAMQWYGPEAGK

Nucleotide

• Length: 423 bp

>T166800 NZ_CP074446:267992-268267 [Klebsiella aerogenes]
ATGGAGCAGCAGGTTTTATCTTTACGTAAAAAACAGCGCAACACGCTGGAGCAGCTGTTTAAAACCCCCGTGCCGCAGGG
TATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGTGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCACCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8122.21 Da
• Isoelectric Point: 8.6591

>AT166800 WP_179182356.1 NZ_CP058556:c4213632-4213417 [Streptomyces sp. CB04723]
MSRTVIDLDDELLADVAQALGTGTKKETVNTALREVLENRRRALALTRLRKAASEGSFDLELFEEKRNYRR

Nucleotide

• Length: 216 bp

>AT166800 NZ_CP074446:268264-268623 [Klebsiella aerogenes]
ATGATCAAACCTAAAACGCCAAATTCAATGGAGATTGCCGGGCAGCCTGCTGTTATTAACTACGTGCCTGAACTCAATGC
ATTCCGCGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGCATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTTGCTGATTGTCGTGAAGCGGGCATCGAGCCTTATGCTCAGCCGGAAAAGCTGAAAACC
TTCACATTGCGCTATCCTGAATCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCAGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA