Detailed information of TA system TA166798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24728..25371
• Replicon: plasmid pS165-1.3
• Accession: NZ_CP058551
• Organism: Klebsiella pneumoniae strain S165-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A5P6KDG5
• Locus tag: HXT63_RS28060
• Protein ID: WP_021312768.1
• Coordinates: 24955..25371 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A5P6KGW8
• Locus tag: HXT63_RS28055
• Protein ID: WP_021312767.1
• Coordinates: 24728..24958 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXT63_RS28020	20005..20871	-	867	WP_004118283.1	replication initiation protein	-
HXT63_RS28025	21406..21510	+	105	WP_032409716.1	hypothetical protein	-
HXT63_RS28030	21639..21896	+	258	WP_000764642.1	hypothetical protein	-
HXT63_RS28035	21954..22730	-	777	WP_049245386.1	site-specific integrase	-
HXT63_RS28040	23034..23417	-	384	WP_063945279.1	hypothetical protein	-
HXT63_RS28045	23575..23880	-	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	-
HXT63_RS28050	23882..24100	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
HXT63_RS28055	24728..24958	+	231	WP_021312767.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HXT63_RS28060	24955..25371	+	417	WP_021312768.1	type II toxin-antitoxin system VapC family toxin	Toxin
HXT63_RS28065	25469..25609	-	141	WP_045286941.1	helix-turn-helix domain-containing protein	-
HXT63_RS28070	25695..26012	-	318	Protein_27	IS6-like element IS26 family transposase	-
HXT63_RS28075	26106..27333	+	1228	Protein_28	IS3-like element ISEc36 family transposase	-
HXT63_RS28080	27345..27734	-	390	Protein_29	IS6-like element IS26 family transposase	-
HXT63_RS28085	27799..29238	+	1440	Protein_30	IS66 family transposase	-
HXT63_RS28090	29274..29798	+	525	Protein_31	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..56644	56644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15175.60 Da
• Isoelectric Point: 7.2452

>T166798 WP_021312768.1 NZ_CP058551:24955-25371 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T166798 NZ_CP074442:c5443075-5442692 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8722.81 Da
• Isoelectric Point: 4.4692

>AT166798 WP_021312767.1 NZ_CP058551:24728-24958 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT166798 NZ_CP074442:c5443317-5443075 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A5P6KDG5

Antitoxin

• AlphaFold DB: A0A5P6KGW8