Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 73529..74054 Replicon plasmid pS165-1.2
Accession NZ_CP058550
Organism Klebsiella pneumoniae strain S165-1

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HXT63_RS27710 Protein ID WP_013023785.1
Coordinates 73749..74054 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HXT63_RS27705 Protein ID WP_001568025.1
Coordinates 73529..73747 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXT63_RS27685 68685..69464 + 780 WP_013023780.1 hypothetical protein -
HXT63_RS27690 69740..71263 + 1524 WP_017899887.1 hypothetical protein -
HXT63_RS27695 71297..72424 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
HXT63_RS27700 72421..72711 + 291 WP_013023783.1 hypothetical protein -
HXT63_RS27705 73529..73747 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HXT63_RS27710 73749..74054 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HXT63_RS27715 74223..74618 + 396 WP_017899885.1 hypothetical protein -
HXT63_RS27720 74645..74959 + 315 WP_053389906.1 hypothetical protein -
HXT63_RS27725 74970..75986 + 1017 WP_017899884.1 hypothetical protein -
HXT63_RS27730 76184..76978 + 795 WP_004197635.1 site-specific integrase -
HXT63_RS27735 77426..77728 - 303 WP_004197636.1 hypothetical protein -
HXT63_RS27740 77725..78351 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1C / blaCTX-M-15 / tet(A) - 1..105000 105000


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T166796 WP_013023785.1 NZ_CP058550:73749-74054 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T166796 NZ_CP074442:c4957411-4957127 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT166796 WP_001568025.1 NZ_CP058550:73529-73747 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT166796 NZ_CP074442:c4957643-4957401 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References