Detailed information of TA system TA166795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 64444..65180
• Replicon: plasmid pS165-1.1
• Accession: NZ_CP058549
• Organism: Klebsiella pneumoniae strain S165-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HXT63_RS26730
• Protein ID: WP_130972229.1
• Coordinates: 64444..64926 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HXT63_RS26735
• Protein ID: WP_003026799.1
• Coordinates: 64914..65180 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXT63_RS26700	59771..59893	+	123	Protein_69	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HXT63_RS26705	59924..60892	+	969	WP_074443085.1	IS5 family transposase	-
HXT63_RS26710	60950..61189	+	240	Protein_71	GNAT family N-acetyltransferase	-
HXT63_RS26715	61218..61915	+	698	WP_231537464.1	IS1 family transposase	-
HXT63_RS26720	61909..62211	-	303	WP_108588601.1	TonB family protein	-
HXT63_RS28295	62595..62726	-	132	WP_004218042.1	hypothetical protein	-
HXT63_RS26725	62887..64233	-	1347	WP_237086710.1	ISNCY family transposase	-
HXT63_RS26730	64444..64926	-	483	WP_130972229.1	GNAT family N-acetyltransferase	Toxin
HXT63_RS26735	64914..65180	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HXT63_RS26740	65835..66059	-	225	WP_237086711.1	hypothetical protein	-
HXT63_RS26745	66034..66327	-	294	WP_227341177.1	hypothetical protein	-
HXT63_RS26750	66358..66930	-	573	WP_227341175.1	hypothetical protein	-
HXT63_RS26755	66927..67175	-	249	WP_227341173.1	hypothetical protein	-
HXT63_RS26760	67794..68210	+	417	WP_100676520.1	hypothetical protein	-
HXT63_RS26765	68263..68943	-	681	WP_049141606.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / dfrA12 / aadA2 / aph(3')-Ia / tet(A)	-	1..169990	169990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17307.89 Da
• Isoelectric Point: 9.8903

>T166795 WP_130972229.1 NZ_CP058549:c64926-64444 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRGYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T166795 NZ_CP074442:c4855567-4855034 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT166795 WP_003026799.1 NZ_CP058549:c65180-64914 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT166795 NZ_CP074442:c4855844-4855578 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66