Detailed information of TA system TA166794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55977..56620
• Replicon: plasmid pS165-1.1
• Accession: NZ_CP058549
• Organism: Klebsiella pneumoniae strain S165-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HXT63_RS26685
• Protein ID: WP_227341187.1
• Coordinates: 56204..56620 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HXT63_RS26680
• Protein ID: WP_119867228.1
• Coordinates: 55977..56207 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXT63_RS26660	51345..52487	-	1143	WP_119867224.1	toll/interleukin-1 receptor domain-containing protein	-
HXT63_RS26665	52621..54237	-	1617	WP_227341191.1	CBASS cGAMP-activated phospholipase	-
HXT63_RS26670	54399..54884	-	486	WP_119867226.1	TIR domain-containing protein	-
HXT63_RS26675	54896..55423	-	528	WP_227341189.1	toll/interleukin-1 receptor domain-containing protein	-
HXT63_RS26680	55977..56207	+	231	WP_119867228.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HXT63_RS26685	56204..56620	+	417	WP_227341187.1	type II toxin-antitoxin system VapC family toxin	Toxin
HXT63_RS26690	56737..57705	-	969	WP_237086708.1	IS5-like element IS903B family transposase	-
HXT63_RS26695	58190..59200	-	1011	WP_237086709.1	hypothetical protein	-
HXT63_RS26700	59771..59893	+	123	Protein_69	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HXT63_RS26705	59924..60892	+	969	WP_074443085.1	IS5 family transposase	-
HXT63_RS26710	60950..61189	+	240	Protein_71	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / dfrA12 / aadA2 / aph(3')-Ia / tet(A)	-	1..169990	169990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15112.60 Da
• Isoelectric Point: 7.8595

>T166794 WP_227341187.1 NZ_CP058549:56204-56620 [Klebsiella pneumoniae]
VNKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNQRIVISAITYAELRFGATGPKASPRHIALVDAFCARLDAVLPWDRA
AVDATTDIKVALRLAGMPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLMLEDWVK

Nucleotide

• Length: 417 bp

>T166794 NZ_CP074442:4242744-4242962 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8682.75 Da
• Isoelectric Point: 4.4563

>AT166794 WP_119867228.1 NZ_CP058549:55977-56207 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFMADREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT166794 NZ_CP074442:4242343-4242717 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG