Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 31187..32097 Replicon plasmid pS165-1.1
Accession NZ_CP058549
Organism Klebsiella pneumoniae strain S165-1

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag HXT63_RS26540 Protein ID WP_004144067.1
Coordinates 31627..32097 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A7H4PRG4
Locus tag HXT63_RS26535 Protein ID WP_032413443.1
Coordinates 31187..31630 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXT63_RS26505 26534..27022 - 489 WP_237086702.1 tyrosine-type recombinase/integrase -
HXT63_RS26510 27180..28148 - 969 WP_237086703.1 IS5-like element IS903B family transposase -
HXT63_RS26515 28168..28353 + 186 WP_071953846.1 DUF2188 domain-containing protein -
HXT63_RS26520 28579..29502 - 924 WP_237086704.1 IS5 family transposase -
HXT63_RS26525 29559..30517 - 959 Protein_34 IS5-like element IS903B family transposase -
HXT63_RS26530 30626..31087 + 462 Protein_35 DUF4113 domain-containing protein -
HXT63_RS26535 31187..31630 + 444 WP_032413443.1 DUF2384 domain-containing protein Antitoxin
HXT63_RS26540 31627..32097 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
HXT63_RS26545 32207..32467 - 261 WP_237086705.1 hypothetical protein -
HXT63_RS26550 33039..33419 - 381 Protein_39 integrase core domain-containing protein -
HXT63_RS26555 33476..34434 - 959 Protein_40 IS5-like element IS903B family transposase -
HXT63_RS26560 34467..35276 - 810 Protein_41 IS481-like element ISKpn28 family transposase -
HXT63_RS26565 35349..35510 + 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
HXT63_RS26570 35746..36714 - 969 WP_237086706.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / floR / dfrA12 / aadA2 / aph(3')-Ia / tet(A) - 1..169990 169990
- inside IScluster/Tn - - 18225..45115 26890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre

(16-147)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T166792 WP_004144067.1 NZ_CP058549:31627-32097 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T166792 NZ_CP074442:2147672-2147774 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16508.82 Da        Isoelectric Point: 10.3013

>AT166792 WP_032413443.1 NZ_CP058549:31187-31630 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT166792 NZ_CP074442:c2147781-2147636 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4PRG4

References