Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2146739..2147364 Replicon chromosome
Accession NZ_CP058548
Organism Klebsiella pneumoniae strain S165-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HXT63_RS10705 Protein ID WP_002882817.1
Coordinates 2146981..2147364 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HXT63_RS10700 Protein ID WP_004150355.1
Coordinates 2146739..2146981 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXT63_RS10675 (2142730) 2142730..2143329 + 600 WP_004151865.1 glucose-1-phosphatase -
HXT63_RS10680 (2143323) 2143323..2144183 + 861 WP_004146232.1 virulence factor BrkB family protein -
HXT63_RS10685 (2144180) 2144180..2144617 + 438 WP_064162494.1 D-aminoacyl-tRNA deacylase -
HXT63_RS10690 (2144662) 2144662..2145603 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HXT63_RS10695 (2145617) 2145617..2146534 - 918 WP_004181612.1 alpha/beta hydrolase -
HXT63_RS10700 (2146739) 2146739..2146981 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HXT63_RS10705 (2146981) 2146981..2147364 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HXT63_RS10710 (2147538) 2147538..2148467 - 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
HXT63_RS10715 (2148464) 2148464..2149099 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HXT63_RS10720 (2149096) 2149096..2149998 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T166786 WP_002882817.1 NZ_CP058548:2146981-2147364 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T166786 NZ_CP074437:3920403-3920888 [Klebsiella sp. A52]
ATGGGAAAAGTAAGCGCGCCGACGCCGCTATCTTCACACCACCAGATTGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTAAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGATAGCC
ACCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCTTTTCACGGACAGGGGCTGGGGGGCGATCT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCTCTGACCG
ATAGCGCGAAACGATTCTATCTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACACTTTTCCTCGCTTTGCCT
AAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT166786 WP_004150355.1 NZ_CP058548:2146739-2146981 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT166786 NZ_CP074437:3920149-3920415 [Klebsiella sp. A52]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCCGCTGAAATTCTGCA
TAAATCGCGGACCGACTTTATTCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGTCGGGTGTTTAACT
TAAGCGATGAACAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References