Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 172068..172978 Replicon plasmid pS161-2.2
Accession NZ_CP058546
Organism Klebsiella pneumoniae strain S161-2

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HYH18_RS27695 Protein ID WP_004026354.1
Coordinates 172068..172538 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HYH18_RS27700 Protein ID WP_004181895.1
Coordinates 172535..172978 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH18_RS27665 167459..167779 - 321 WP_040150694.1 transcriptional regulator -
HYH18_RS27670 167865..168563 + 699 WP_040150695.1 arsenical resistance protein ArsH -
HYH18_RS27675 168572..169486 - 915 Protein_184 IS3 family transposase -
HYH18_RS27680 169494..170462 + 969 WP_077254853.1 IS5-like element IS903B family transposase -
HYH18_RS27685 170632..171003 - 372 WP_194433529.1 helix-turn-helix domain-containing protein -
HYH18_RS27690 171698..171958 + 261 WP_004026352.1 hypothetical protein -
HYH18_RS27695 172068..172538 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HYH18_RS27700 172535..172978 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HYH18_RS27705 173079..173756 - 678 Protein_190 DUF4113 domain-containing protein -
HYH18_RS27710 173825..174607 - 783 WP_032437422.1 IS21-like element ISSen3 family helper ATPase IstB -
HYH18_RS27715 174604..175626 - 1023 WP_129304158.1 IS21-like element ISSen3 family transposase -
HYH18_RS27720 175714..176039 + 326 Protein_193 transposase -
HYH18_RS27725 176096..176296 - 201 Protein_194 integrase core domain-containing protein -
HYH18_RS27730 176756..177440 - 685 Protein_195 IS1 family transposase -
HYH18_RS27735 177519..177698 + 180 WP_007372130.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / blaDHA-1 / qnrB4 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..178411 178411
- inside IScluster/Tn - - 161981..176965 14984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T166773 WP_004026354.1 NZ_CP058546:c172538-172068 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T166773 NZ_CP074428:c4072898-4072614 [Salmonella sp. SJTUF14523]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT166773 WP_004181895.1 NZ_CP058546:c172978-172535 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT166773 NZ_CP074428:c4073130-4072888 [Salmonella sp. SJTUF14523]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References