Detailed information of TA system TA166770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 5269088..5269898
• Replicon: chromosome
• Accession: NZ_CP058544
• Organism: Klebsiella pneumoniae strain S161-2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: HYH18_RS25820
• Protein ID: WP_014908042.1
• Coordinates: 5269088..5269621 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HYH18_RS25825
• Protein ID: WP_023343051.1
• Coordinates: 5269632..5269898 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH18_RS25815	5267919..5269040	+	1122	WP_237085237.1	cupin domain-containing protein	-
HYH18_RS25820	5269088..5269621	-	534	WP_014908042.1	type II toxin-antitoxin system toxin KacT	Toxin
HYH18_RS25825	5269632..5269898	-	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
HYH18_RS25830	5270001..5271434	-	1434	WP_094310098.1	Cu(+)/Ag(+) sensor histidine kinase	-
HYH18_RS25835	5271424..5272107	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HYH18_RS25840	5272280..5273665	+	1386	WP_094310099.1	efflux transporter outer membrane subunit	-
HYH18_RS25845	5273683..5274027	+	345	WP_004214146.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T166770 WP_014908042.1 NZ_CP058544:c5269621-5269088 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T166770 NZ_CP074428:3602463-3602720 [Salmonella sp. SJTUF14523]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT166770 WP_023343051.1 NZ_CP058544:c5269898-5269632 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT166770 NZ_CP074428:c3602638-3602306 [Salmonella sp. SJTUF14523]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A332NV98