Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2398140..2398730 Replicon chromosome
Accession NZ_CP058544
Organism Klebsiella pneumoniae strain S161-2

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag HYH18_RS11575 Protein ID WP_022615588.1
Coordinates 2398398..2398730 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag HYH18_RS11570 Protein ID WP_000288812.1
Coordinates 2398140..2398397 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH18_RS11545 2393233..2394211 + 979 Protein_2240 IS5-like element ISKpn26 family transposase -
HYH18_RS11550 2394475..2396223 + 1749 WP_075201963.1 hypothetical protein -
HYH18_RS11555 2396302..2396859 + 558 WP_040245065.1 hypothetical protein -
HYH18_RS11560 2397142..2397602 + 461 Protein_2243 hypothetical protein -
HYH18_RS11565 2397599..2397805 + 207 WP_020805021.1 helix-turn-helix transcriptional regulator -
HYH18_RS11570 2398140..2398397 + 258 WP_000288812.1 antitoxin Antitoxin
HYH18_RS11575 2398398..2398730 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HYH18_RS11585 2399052..2400488 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HYH18_RS11595 2400854..2402308 - 1455 WP_094310693.1 AMP nucleosidase -
HYH18_RS11600 2402438..2402683 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T166758 WP_022615588.1 NZ_CP058544:2398398-2398730 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T166758 NZ_CP074426:60142-60423 [Salmonella sp. SJTUF15034]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT166758 WP_000288812.1 NZ_CP058544:2398140-2398397 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT166758 NZ_CP074426:59901-60152 [Salmonella sp. SJTUF15034]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References