Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 561117..561742 Replicon chromosome
Accession NZ_CP058544
Organism Klebsiella pneumoniae strain S161-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HYH18_RS02650 Protein ID WP_002882817.1
Coordinates 561117..561500 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HYH18_RS02655 Protein ID WP_004150355.1
Coordinates 561500..561742 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH18_RS02635 558483..559385 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HYH18_RS02640 559382..560017 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HYH18_RS02645 560014..560943 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HYH18_RS02650 561117..561500 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HYH18_RS02655 561500..561742 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HYH18_RS02660 561947..562864 + 918 WP_032422045.1 alpha/beta hydrolase -
HYH18_RS02665 562878..563819 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HYH18_RS02670 563864..564301 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HYH18_RS02675 564298..565158 - 861 WP_004146232.1 virulence factor BrkB family protein -
HYH18_RS02680 565152..565751 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T166753 WP_002882817.1 NZ_CP058544:c561500-561117 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T166753 NZ_CP074425:c4072333-4072049 [Salmonella sp. SJTUF15034]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT166753 WP_004150355.1 NZ_CP058544:c561742-561500 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT166753 NZ_CP074425:c4072565-4072323 [Salmonella sp. SJTUF15034]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References