Detailed information of TA system TA166750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3704261..3704854
• Replicon: chromosome
• Accession: NZ_CP058533
• Organism: Pseudomonas fluorescens strain W-6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HXS75_RS16740
• Protein ID: WP_032890654.1
• Coordinates: 3704261..3704560 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A031IS10
• Locus tag: HXS75_RS16745
• Protein ID: WP_032890601.1
• Coordinates: 3704564..3704854 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS75_RS16715	3699443..3700069	-	627	WP_032890608.1	precorrin-8X methylmutase	-
HXS75_RS16720	3700062..3701324	-	1263	WP_179180102.1	precorrin-3B synthase	-
HXS75_RS16725	3701447..3702652	+	1206	WP_179179574.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
HXS75_RS16730	3702649..3703362	+	714	WP_179179575.1	cobalt-precorrin-6A reductase	-
HXS75_RS16735	3703488..3703739	-	252	WP_032890602.1	hypothetical protein	-
HXS75_RS16740	3704261..3704560	+	300	WP_032890654.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HXS75_RS16745	3704564..3704854	+	291	WP_032890601.1	putative addiction module antidote protein	Antitoxin
HXS75_RS16750	3704952..3705347	+	396	WP_095186039.1	DUF2946 domain-containing protein	-
HXS75_RS16755	3705395..3705877	+	483	WP_179179576.1	copper chaperone PCu(A)C	-
HXS75_RS16760	3705889..3706302	+	414	WP_095186040.1	DUF2946 domain-containing protein	-
HXS75_RS16765	3706396..3708462	+	2067	WP_179179577.1	TonB-dependent copper receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11482.08 Da
• Isoelectric Point: 6.9822

>T166750 WP_032890654.1 NZ_CP058533:3704261-3704560 [Pseudomonas fluorescens]
IEFNETTEFVKWLEAVRDPFVKVRVIKRVRMAEAGNFGDCESVGDSVFEMRIHYGPGYRVYFTRRDEVVYLLLIGGDKST
QSRDIKRAKQIAINFGSEE

Nucleotide

• Length: 300 bp

>T166750 NZ_CP074425:3601897-3602154 [Salmonella sp. SJTUF15034]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10417.99 Da
• Isoelectric Point: 5.8124

>AT166750 WP_032890601.1 NZ_CP058533:3704564-3704854 [Pseudomonas fluorescens]
MSESFTRFDAAKYLKTPLEMAAYLETCFEDDAGDGLLIRTALNDIARAQGMTQVARDAGLGRESLYKALSSNGNPEFATI
MKVMKALGLKLHATAI

Nucleotide

• Length: 291 bp

>AT166750 NZ_CP074425:c3602072-3601740 [Salmonella sp. SJTUF15034]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Antitoxin

• AlphaFold DB: A0A031IS10