Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 170130..170761 Replicon plasmid pAtEML4
Accession NZ_CP058527
Organism Agrobacterium tumefaciens strain EML4

Toxin (Protein)


Gene name vapC Uniprot ID A0A822VAP2
Locus tag EML4_RS24305 Protein ID WP_060725879.1
Coordinates 170360..170761 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A822VB92
Locus tag EML4_RS24300 Protein ID WP_060725878.1
Coordinates 170130..170360 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EML4_RS24275 165556..165885 + 330 WP_121593591.1 nuclear transport factor 2 family protein -
EML4_RS24280 165882..166661 + 780 WP_121593590.1 SDR family oxidoreductase -
EML4_RS24285 166688..167155 - 468 WP_121593589.1 MarR family transcriptional regulator -
EML4_RS24290 167300..168094 + 795 WP_121593588.1 alpha/beta hydrolase -
EML4_RS24295 168287..169918 + 1632 WP_121593587.1 GMC family oxidoreductase -
EML4_RS24300 170130..170360 + 231 WP_060725878.1 antitoxin Antitoxin
EML4_RS24305 170360..170761 + 402 WP_060725879.1 tRNA(fMet)-specific endonuclease VapC Toxin
EML4_RS24310 170837..171067 - 231 WP_060726006.1 BrnA antitoxin family protein -
EML4_RS24315 171304..171948 - 645 WP_060725880.1 LysE family translocator -
EML4_RS24320 172060..172890 - 831 WP_121593586.1 AraC family transcriptional regulator -
EML4_RS24325 173799..174359 - 561 WP_162993134.1 TetR/AcrR family transcriptional regulator -
EML4_RS24330 174469..175257 + 789 WP_121593584.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..520191 520191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 17193.34 Da        Isoelectric Point: 12.9238

>T166747 WP_060725879.1 NZ_CP058527:170360-170761 [Agrobacterium tumefaciens]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Download         Length: 402 bp

>T166747 NZ_CP074425:c2443574-2443419 [Salmonella sp. SJTUF15034]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 9887.42 Da        Isoelectric Point: 12.6808

>AT166747 WP_060725878.1 NZ_CP058527:170130-170360 [Agrobacterium tumefaciens]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Download         Length: 231 bp

>AT166747 NZ_CP074425:2443586-2443644 [Salmonella sp. SJTUF15034]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822VAP2

Antitoxin

Source ID Structure
AlphaFold DB A0A822VB92

References