Detailed information of TA system TA166746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 234685..235187
• Replicon: plasmid pIAE1
• Accession: NZ_CP058410
• Organism: Trichlorobacter lovleyi strain IAE

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FY034_RS18640
• Protein ID: WP_265552565.1
• Coordinates: 234685..234939 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: FY034_RS18645
• Protein ID: WP_265555334.1
• Coordinates: 234936..235187 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY034_RS18615 (FY034_18635)	229815..230381	+	567	WP_265555395.1	guanylate kinase	-
FY034_RS18620 (FY034_18640)	230385..230963	+	579	WP_265555398.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
FY034_RS18625 (FY034_18645)	230967..231983	-	1017	WP_265555400.1	FAD:protein FMN transferase	-
FY034_RS18630 (FY034_18650)	232577..233914	-	1338	WP_224963446.1	transposase	-
FY034_RS18635 (FY034_18655)	234146..234643	+	498	WP_265555332.1	alpha/beta hydrolase	-
FY034_RS18640 (FY034_18660)	234685..234939	-	255	WP_265552565.1	Txe/YoeB family addiction module toxin	Toxin
FY034_RS18645 (FY034_18665)	234936..235187	-	252	WP_265555334.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FY034_RS18650 (FY034_18670)	235313..236254	+	942	WP_265555403.1	adenosylcobinamide-phosphate synthase CbiB	-
FY034_RS18655 (FY034_18675)	236514..237644	+	1131	WP_265555404.1	ATP-binding protein	-
FY034_RS18660 (FY034_18680)	237811..238524	-	714	WP_265555406.1	NAD-dependent epimerase/dehydratase family protein	-
FY034_RS18665 (FY034_18685)	238503..238862	-	360	Protein_272	GNAT family N-acetyltransferase	-
FY034_RS18670 (FY034_18690)	238990..239829	-	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
FY034_RS18675 (FY034_18695)	239823..240170	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE	-	1..289634	289634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9956.52 Da
• Isoelectric Point: 8.4984

>T166746 WP_265552565.1 NZ_CP058410:c234939-234685 [Trichlorobacter lovleyi]
MMIKFSTAAWEDYLYWQTTDKAMLKRINQLIRDIQREPFAGIGKPEPLKHQLAGFWSRRIDSCHRLVYAVEGDTLLIAQC
RDHY

Nucleotide

• Length: 255 bp

>T166746 NZ_CP074425:c988502-987975 [Salmonella sp. SJTUF15034]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9391.56 Da
• Isoelectric Point: 5.5900

>AT166746 WP_265555334.1 NZ_CP058410:c235187-234936 [Trichlorobacter lovleyi]
MNALTYTYTRQHFAEVMKSVNEDHVPVVVTSQRGKPVVILSLDDFHAYEETAYLLRNPQGAKRLLESVEELRAGGGQTRD
LTE

Nucleotide

• Length: 252 bp

>AT166746 NZ_CP074425:c988789-988499 [Salmonella sp. SJTUF15034]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA