Detailed information of TA system TA166745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 172498..172991
• Replicon: plasmid pIAE1
• Accession: NZ_CP058410
• Organism: Trichlorobacter lovleyi strain IAE

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FY034_RS18270
• Protein ID: WP_265552565.1
• Coordinates: 172498..172752 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: FY034_RS18275
• Protein ID: WP_265555733.1
• Coordinates: 172749..172991 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY034_RS18240 (FY034_18265)	167956..168363	+	408	WP_265555340.1	pyridoxamine 5'-phosphate oxidase family protein	-
FY034_RS18245 (FY034_18270)	168457..168792	+	336	WP_265555342.1	DUF1516 family protein	-
FY034_RS18250 (FY034_18275)	168893..169846	+	954	WP_265555344.1	glutathione synthase	-
FY034_RS18255 (FY034_18280)	169843..170409	+	567	WP_265555346.1	hypothetical protein	-
FY034_RS18260 (FY034_18285)	170390..171727	-	1338	WP_224963446.1	transposase	-
FY034_RS18265 (FY034_18290)	171959..172456	+	498	WP_265555332.1	alpha/beta hydrolase	-
FY034_RS18270 (FY034_18295)	172498..172752	-	255	WP_265552565.1	Txe/YoeB family addiction module toxin	Toxin
FY034_RS18275 (FY034_18300)	172749..172991	-	243	WP_265555733.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FY034_RS18280 (FY034_18305)	172991..173308	-	318	Protein_196	conjugal transfer protein TrbE	-
FY034_RS18285 (FY034_18310)	173321..173593	-	273	WP_004883097.1	VirB3 family type IV secretion system protein	-
FY034_RS18290 (FY034_18315)	173590..173982	-	393	WP_041111012.1	TrbC/VirB2 family protein	-
FY034_RS18295 (FY034_18320)	173979..175046	-	1068	WP_041111014.1	P-type conjugative transfer ATPase TrbB	-
FY034_RS18300 (FY034_18325)	175043..175519	-	477	WP_041111016.1	ribbon-helix-helix protein, CopG family	-
FY034_RS18305 (FY034_18330)	175516..177195	-	1680	Protein_201	conjugal transfer protein TraG	-
FY034_RS18310 (FY034_18335)	177194..177778	-	585	Protein_202	type II secretion system protein GspD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE	-	1..289634	289634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-71)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9956.52 Da
• Isoelectric Point: 8.4984

>T166745 WP_265552565.1 NZ_CP058410:c172752-172498 [Trichlorobacter lovleyi]
MMIKFSTAAWEDYLYWQTTDKAMLKRINQLIRDIQREPFAGIGKPEPLKHQLAGFWSRRIDSCHRLVYAVEGDTLLIAQC
RDHY

Nucleotide

• Length: 255 bp

>T166745 NZ_CP074425:836174-836572 [Salmonella sp. SJTUF15034]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9093.22 Da
• Isoelectric Point: 5.5900

>AT166745 WP_265555733.1 NZ_CP058410:c172991-172749 [Trichlorobacter lovleyi]
MTYTYTRQHFAEVMKSVNEDHVPVVVTSQRGKPVVILSLDDFHAYEETAYLLRNPQGAKRLLESVEELRAGGGQTRDLTE

Nucleotide

• Length: 243 bp

>AT166745 NZ_CP074425:835947-836174 [Salmonella sp. SJTUF15034]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA