Detailed information of TA system TA166736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53886..54622
• Replicon: plasmid unnamed1
• Accession: NZ_CP058362
• Organism: Klebsiella pneumoniae strain KP77

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HYD36_RS26475
• Protein ID: WP_003026803.1
• Coordinates: 54140..54622 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HYD36_RS26470
• Protein ID: WP_003026799.1
• Coordinates: 53886..54152 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYD36_RS26425 (HYD36_26410)	49948..50310	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
HYD36_RS26430 (HYD36_26415)	50360..50710	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HYD36_RS26435 (HYD36_26420)	51068..51337	+	270	WP_004152102.1	hypothetical protein	-
HYD36_RS26440 (HYD36_26425)	51325..51900	+	576	WP_004152103.1	hypothetical protein	-
HYD36_RS26445 (HYD36_26430)	51931..52425	+	495	WP_004152104.1	DNA-binding protein	-
HYD36_RS26450 (HYD36_26435)	52469..52837	+	369	WP_004152105.1	hypothetical protein	-
HYD36_RS26455 (HYD36_26440)	52871..53074	+	204	WP_004152106.1	HHA domain-containing protein	-
HYD36_RS26460 (HYD36_26445)	53123..53380	+	258	WP_004152107.1	hypothetical protein	-
HYD36_RS26465 (HYD36_26450)	53456..53710	+	255	WP_004152108.1	hypothetical protein	-
HYD36_RS26470 (HYD36_26455)	53886..54152	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HYD36_RS26475 (HYD36_26460)	54140..54622	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HYD36_RS26480 (HYD36_26465)	54781..55946	-	1166	Protein_59	IS3 family transposase	-
HYD36_RS26485 (HYD36_26470)	56123..57085	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
HYD36_RS26490 (HYD36_26475)	57072..57560	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-
HYD36_RS26495 (HYD36_26480)	58058..58255	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C	-	1..191595	191595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T166736 WP_003026803.1 NZ_CP058362:54140-54622 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T166736 NZ_CP074415:2100624-2100914 [Vibrio parahaemolyticus]
ATGACCTATAAACTCGACTTTAAAAAGAGCGCCCTCAAAGAGTGGAAAAAGCTTGGTTCTACTCTGCAACAACAATTTAA
GAAAAAGCTAATCGAGCGCTTAGATAACCCACATGTTCCGGCTTCAAAACTATCTGGAGCTGACAACATGTATAAAATTA
AGTTGCGTCAATCGGGCTACCGTCTCGTCTACAAAGTTGAAGACGATGTCATCATTGTAACCGTCTTAGCAGTCGGAAAA
CGCGAACGTAGCGATGTTTACCGTAAATCCATGAAAAGGTTAGATAATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT166736 WP_003026799.1 NZ_CP058362:53886-54152 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT166736 NZ_CP074415:2100386-2100634 [Vibrio parahaemolyticus]
ATGACCACTAGAATTTTAGCCGATGTTGCTGCAAGCATTACTGAGTTGAAAGCTAACCCTATGAAAGTTGCAACTAGTGC
TTACGGCGAACCTGTTGCTGTATTAAACCGAAATGAGCCAGCATTCTATTGCGTACCTGCCGAAGCGTACGAAATGATGA
TGGACAGACTCGAAGATCTTGAACTACTCGCTATCGCTAAAGAGCGTGAATCTGAAGAGAGCATTTCGGTAAATATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66