Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5286543..5287168 Replicon chromosome
Accession NZ_CP058361
Organism Klebsiella pneumoniae strain KP77

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HYD36_RS25825 Protein ID WP_002882817.1
Coordinates 5286543..5286926 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HYD36_RS25830 Protein ID WP_004150355.1
Coordinates 5286926..5287168 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD36_RS25810 (5283909) 5283909..5284811 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HYD36_RS25815 (5284808) 5284808..5285443 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HYD36_RS25820 (5285440) 5285440..5286369 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HYD36_RS25825 (5286543) 5286543..5286926 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HYD36_RS25830 (5286926) 5286926..5287168 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HYD36_RS25835 (5287373) 5287373..5288290 + 918 WP_023286983.1 alpha/beta hydrolase -
HYD36_RS25840 (5288304) 5288304..5289245 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HYD36_RS25845 (5289290) 5289290..5289727 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HYD36_RS25850 (5289724) 5289724..5290584 - 861 WP_004146232.1 virulence factor BrkB family protein -
HYD36_RS25855 (5290578) 5290578..5291177 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T166735 WP_002882817.1 NZ_CP058361:c5286926-5286543 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T166735 NZ_CP074412:5990370-5990657 [Pseudomonas viridiflava]
GTGACGACAGTTGAATGGAGTTCTTGGGCCAGGCAGGACTTGCTGGCGATCATCGATTACATCTCTGATGACAACGTGAC
GGCGGCGCAAGAAATGAAGGACACCATCGAAAGCCGGGTGGCGGATCTTCCTTTGCATCCAAGGCTTTATAAGGAAGGCC
GTGAGCCGGGGACCCGCGAGATGGTCGTACATCCAAATTACATCGTTATCTATAGTGAGAGAACGACAACGCTGATAGTG
CTGCGGGTGCTACATGTCGCTCGAAAGTGGCCCTCCGAAACCGAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT166735 WP_004150355.1 NZ_CP058361:c5287168-5286926 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT166735 NZ_CP074412:5990084-5990383 [Pseudomonas viridiflava]
ATGGCAACGCACACATCCATGCTGCATGTACGTATAGATAACGATCTCAAAACACAGGCGACCGAAACGCTAGCCAAATT
CGGCCTTACCGTGTCGGATGCGGTCAGGATTCTGCTGACCCGTGTCGCCAGGGAAGGTGCCCTGCCTGTCGGGTTCACCA
GCGATGCCGAAGCGTACGACAAGTGGTTTCGGGCAAAGGTAAAAGAAGCCATGGATGACACGCAACCGTCCATAACTCAC
GAACAGATGATGGCAGAAGCTCAGCAGCTAATAGACAGGAAGCGCCGTGACGACAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References