Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4668532..4669342 Replicon chromosome
Accession NZ_CP058361
Organism Klebsiella pneumoniae strain KP77

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HYD36_RS22890 Protein ID WP_002887280.1
Coordinates 4668532..4669065 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HYD36_RS22895 Protein ID WP_002887278.1
Coordinates 4669076..4669342 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD36_RS22885 (4667363) 4667363..4668484 + 1122 WP_009309849.1 cupin domain-containing protein -
HYD36_RS22890 (4668532) 4668532..4669065 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HYD36_RS22895 (4669076) 4669076..4669342 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HYD36_RS22900 (4669445) 4669445..4670878 - 1434 WP_023287082.1 Cu(+)/Ag(+) sensor histidine kinase -
HYD36_RS22905 (4670868) 4670868..4671551 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HYD36_RS22910 (4671724) 4671724..4673109 + 1386 WP_032414381.1 efflux transporter outer membrane subunit -
HYD36_RS22915 (4673127) 4673127..4673471 + 345 WP_023287080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T166732 WP_002887280.1 NZ_CP058361:c4669065-4668532 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T166732 NZ_CP074412:c4129810-4129529 [Pseudomonas viridiflava]
ATGTTGCCCATTGTCTGGCTGCAAGCTGCTGTAAATGACCTTTCTTCAATCATCCTCTACATCGCCAATGAAAACCCCTC
TGCCGCTCGTCGCCTAAAATCACGTCTTCAAGCTGCGCCTTTACCATTAGCTGAACATCCTTATCTTTTTCCTCTGGGGC
GGGTTTCCGGTACGCGCGAACTCGTCGCGCACCCCAATTACATCATCGTTTACCGCATTGCCTCAGATCGAGTGGAGATC
GTGAACGTCTTGCATTCAAGGCAGACCTATCCGTTGACATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT166732 WP_002887278.1 NZ_CP058361:c4669342-4669076 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT166732 NZ_CP074412:c4130004-4129810 [Pseudomonas viridiflava]
ATGAGCACTACACTTTCTCCCATCGTTTCCGAATTTGATACGCAAGAGCAAGCTGATAGCTACGATCTCTGGTTTCGCGC
CAAGGTGGAAGCTTCGTTGGCCGAACCTGGACGTGCAGTGCCTCACGATGAAGTCATGGCGCGCATGGACGCGATCATTG
AGGCTGCTGAGGCCAGGCAGAGATCGGGTAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References