Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1719211..1719801 Replicon chromosome
Accession NZ_CP058361
Organism Klebsiella pneumoniae strain KP77

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag HYD36_RS08310 Protein ID WP_032421545.1
Coordinates 1719469..1719801 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag HYD36_RS08305 Protein ID WP_096106567.1
Coordinates 1719211..1719468 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD36_RS28075 (1715127) 1715127..1715204 + 78 Protein_1616 type II toxin-antitoxin system PemK/MazF family toxin -
HYD36_RS08285 (1715555) 1715555..1717303 + 1749 WP_096106564.1 hypothetical protein -
HYD36_RS08290 (1717382) 1717382..1717930 + 549 WP_096106565.1 hypothetical protein -
HYD36_RS08295 (1718213) 1718213..1718673 + 461 Protein_1619 hypothetical protein -
HYD36_RS08300 (1718670) 1718670..1718876 + 207 WP_096106566.1 helix-turn-helix transcriptional regulator -
HYD36_RS08305 (1719211) 1719211..1719468 + 258 WP_096106567.1 antitoxin Antitoxin
HYD36_RS08310 (1719469) 1719469..1719801 + 333 WP_032421545.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HYD36_RS08320 (1720123) 1720123..1721559 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HYD36_RS08330 (1721925) 1721925..1723379 - 1455 WP_023286675.1 AMP nucleosidase -
HYD36_RS08335 (1723509) 1723509..1723754 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11917.79 Da        Isoelectric Point: 10.0968

>T166721 WP_032421545.1 NZ_CP058361:1719469-1719801 [Klebsiella pneumoniae]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T166721 NZ_CP074409:c931600-931196 [Pseudomonas syringae]
ATGCTCAAGTACATGCTTGACACCAATATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCACGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCCG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGGGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATCACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTGAGGGTAGAAGACTGGGTAAGCC
AATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9189.65 Da        Isoelectric Point: 4.7468

>AT166721 WP_096106567.1 NZ_CP058361:1719211-1719468 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLGLSTDSEVGMTIDNGCLVIEPQKRPHYSLEELLAQCDPHAEISEGGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT166721 NZ_CP074409:c931830-931600 [Pseudomonas syringae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTGGCCTTGCCAGACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCTGCCGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGTGGACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References