Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1940..2499 Replicon plasmid unnamed2
Accession NZ_CP058360
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag HYD35_RS27545 Protein ID WP_000421257.1
Coordinates 2224..2499 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag HYD35_RS27540 Protein ID WP_001178089.1
Coordinates 1940..2224 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS27535 (HYD35_27530) 1..1032 + 1032 WP_000907880.1 plasmid replication initiator RepA -
HYD35_RS27540 (HYD35_27535) 1940..2224 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
HYD35_RS27545 (HYD35_27540) 2224..2499 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HYD35_RS27550 (HYD35_27545) 2605..2886 + 282 WP_001105066.1 hypothetical protein -
HYD35_RS27555 (HYD35_27550) 3077..3343 - 267 WP_000627831.1 hypothetical protein -
HYD35_RS27565 (HYD35_27560) 3581..4183 - 603 WP_000517694.1 ProQ/FINO family protein -
HYD35_RS27570 (HYD35_27565) 4537..5883 + 1347 WP_001132025.1 protein YagA -
HYD35_RS27575 (HYD35_27570) 6410..6712 - 303 WP_001245884.1 hypothetical protein -
HYD35_RS27580 (HYD35_27575) 6709..7335 - 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / blaTEM-1C / aph(3'')-Ib / sul2 - 1..103005 103005


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T166718 WP_000421257.1 NZ_CP058360:2224-2499 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T166718 NZ_CP074407:c4236168-4235788 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT166718 WP_001178089.1 NZ_CP058360:1940-2224 [Klebsiella pneumoniae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT166718 NZ_CP074407:c4236589-4236215 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References