Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53886..54622 Replicon plasmid unnamed1
Accession NZ_CP058359
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HYD35_RS26950 Protein ID WP_003026803.1
Coordinates 54140..54622 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HYD35_RS26945 Protein ID WP_003026799.1
Coordinates 53886..54152 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS26900 (HYD35_26890) 49948..50310 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
HYD35_RS26905 (HYD35_26895) 50360..50710 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HYD35_RS26910 (HYD35_26900) 51068..51337 + 270 WP_004152102.1 hypothetical protein -
HYD35_RS26915 (HYD35_26905) 51325..51900 + 576 WP_004152103.1 hypothetical protein -
HYD35_RS26920 (HYD35_26910) 51931..52425 + 495 WP_004152104.1 DNA-binding protein -
HYD35_RS26925 (HYD35_26915) 52469..52837 + 369 WP_004152105.1 hypothetical protein -
HYD35_RS26930 (HYD35_26920) 52871..53074 + 204 WP_004152106.1 HHA domain-containing protein -
HYD35_RS26935 (HYD35_26925) 53123..53380 + 258 WP_004152107.1 hypothetical protein -
HYD35_RS26940 (HYD35_26930) 53456..53710 + 255 WP_004152108.1 hypothetical protein -
HYD35_RS26945 (HYD35_26935) 53886..54152 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HYD35_RS26950 (HYD35_26940) 54140..54622 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HYD35_RS26955 (HYD35_26945) 54781..55946 - 1166 Protein_59 IS3 family transposase -
HYD35_RS26960 (HYD35_26950) 56123..57085 - 963 WP_004152113.1 zinc metalloprotease HtpX -
HYD35_RS26965 (HYD35_26955) 57072..57560 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -
HYD35_RS26970 (HYD35_26960) 58058..58255 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1C - 1..161606 161606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T166717 WP_003026803.1 NZ_CP058359:54140-54622 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T166717 NZ_CP074407:4077394-4077501 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT166717 WP_003026799.1 NZ_CP058359:53886-54152 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT166717 NZ_CP074407:c4077344-4077281 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References