Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5374111..5374736 Replicon chromosome
Accession NZ_CP058358
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HYD35_RS26300 Protein ID WP_002882817.1
Coordinates 5374111..5374494 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HYD35_RS26305 Protein ID WP_004150355.1
Coordinates 5374494..5374736 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS26285 (5371477) 5371477..5372379 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HYD35_RS26290 (5372376) 5372376..5373011 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HYD35_RS26295 (5373008) 5373008..5373937 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HYD35_RS26300 (5374111) 5374111..5374494 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HYD35_RS26305 (5374494) 5374494..5374736 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HYD35_RS26310 (5374941) 5374941..5375858 + 918 WP_023286983.1 alpha/beta hydrolase -
HYD35_RS26315 (5375872) 5375872..5376813 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HYD35_RS26320 (5376858) 5376858..5377295 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HYD35_RS26325 (5377292) 5377292..5378152 - 861 WP_004146232.1 virulence factor BrkB family protein -
HYD35_RS26330 (5378146) 5378146..5378745 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T166716 WP_002882817.1 NZ_CP058358:c5374494-5374111 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T166716 NZ_CP074407:4076859-4076966 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT166716 WP_004150355.1 NZ_CP058358:c5374736-5374494 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT166716 NZ_CP074407:c4076812-4076746 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References