Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4756100..4756910 Replicon chromosome
Accession NZ_CP058358
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HYD35_RS23365 Protein ID WP_002887280.1
Coordinates 4756100..4756633 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HYD35_RS23370 Protein ID WP_002887278.1
Coordinates 4756644..4756910 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS23360 (4754931) 4754931..4756052 + 1122 WP_009309849.1 cupin domain-containing protein -
HYD35_RS23365 (4756100) 4756100..4756633 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HYD35_RS23370 (4756644) 4756644..4756910 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HYD35_RS23375 (4757013) 4757013..4758446 - 1434 WP_023287082.1 Cu(+)/Ag(+) sensor histidine kinase -
HYD35_RS23380 (4758436) 4758436..4759119 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HYD35_RS23385 (4759292) 4759292..4760677 + 1386 WP_032414381.1 efflux transporter outer membrane subunit -
HYD35_RS23390 (4760695) 4760695..4761039 + 345 WP_023287080.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T166713 WP_002887280.1 NZ_CP058358:c4756633-4756100 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T166713 NZ_CP074407:3373932-3374035 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT166713 WP_002887278.1 NZ_CP058358:c4756910-4756644 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT166713 NZ_CP074407:c3374073-3373802 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References