Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4156870..4157489 Replicon chromosome
Accession NZ_CP058358
Organism Klebsiella pneumoniae strain KP486

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HYD35_RS20500 Protein ID WP_002892050.1
Coordinates 4157271..4157489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HYD35_RS20495 Protein ID WP_002892066.1
Coordinates 4156870..4157244 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD35_RS20485 (4152022) 4152022..4153215 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HYD35_RS20490 (4153238) 4153238..4156384 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HYD35_RS20495 (4156870) 4156870..4157244 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HYD35_RS20500 (4157271) 4157271..4157489 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HYD35_RS20505 (4157648) 4157648..4158214 + 567 WP_002892030.1 maltose O-acetyltransferase -
HYD35_RS20510 (4158186) 4158186..4158326 - 141 WP_004147370.1 hypothetical protein -
HYD35_RS20515 (4158347) 4158347..4158817 + 471 WP_002892026.1 YlaC family protein -
HYD35_RS20520 (4158792) 4158792..4160243 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HYD35_RS20525 (4160344) 4160344..4161042 + 699 WP_002892021.1 GNAT family protein -
HYD35_RS20530 (4161039) 4161039..4161179 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HYD35_RS20535 (4161179) 4161179..4161442 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166712 WP_002892050.1 NZ_CP058358:4157271-4157489 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T166712 NZ_CP074407:3180771-3181025 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CTTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT166712 WP_002892066.1 NZ_CP058358:4156870-4157244 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT166712 NZ_CP074407:3180523-3180774 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References