Detailed information of TA system TA166691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 29907..30705
• Replicon: plasmid pAMPD1
• Accession: NZ_CP058356
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HXS77_RS22250
• Protein ID: WP_032187665.1
• Coordinates: 30184..30705 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: HXS77_RS22245
• Protein ID: WP_001711191.1
• Coordinates: 29907..30176 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS22220	25333..26673	-	1341	WP_000137333.1	AAA family ATPase	-
HXS77_RS22225	26717..27457	-	741	WP_001717320.1	hypothetical protein	-
HXS77_RS22230	27740..28507	+	768	WP_000342417.1	hypothetical protein	-
HXS77_RS22235	28560..28913	-	354	WP_160378290.1	hypothetical protein	-
HXS77_RS22240	28919..29587	-	669	WP_000161229.1	AAA family ATPase	-
HXS77_RS22245	29907..30176	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
HXS77_RS22250	30184..30705	+	522	WP_032187665.1	GNAT family N-acetyltransferase	Toxin
HXS77_RS22255	30874..31125	-	252	WP_077108702.1	hypothetical protein	-
HXS77_RS22260	31127..31819	-	693	WP_012640731.1	hypothetical protein	-
HXS77_RS22265	31833..32156	-	324	WP_000064175.1	hypothetical protein	-
HXS77_RS22270	32231..33013	-	783	WP_077108701.1	receptor-recognizing protein	-
HXS77_RS22275	33080..33871	-	792	Protein_34	tail fiber domain-containing protein	-
HXS77_RS22280	34502..35299	-	798	Protein_35	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112466	112466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19345.11 Da
• Isoelectric Point: 7.7881

>T166691 WP_032187665.1 NZ_CP058356:30184-30705 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T166691 NZ_CP074393:c597684-597349 [Agrobacterium salinitolerans]
GTGACGGATAAAAAAGGTCACGGAAAAGACGCGGCATCGAAACGAACGGTGTTGCCGCGTAGTTCGGATTTCACCAGGCA
ATTTATCAAGGACTGGCAACGGCTGAACAATTCCGGGCGCTATGACATGGTGAAACTGAAGGAGATCATGCTCCTTCTCA
TCGCCGGCGAAGCGCCATTACCGGCGCAGTTTCGTGACCATGAACTGAACGGAGAATGGCACGATCATCGCGAATGCCAT
GTCGGCGGGGACTTTCTGTTGATCTATAAACTTGATGAGAAGCAGAACCTTCTCATCTTCACCCGCGCAGGAACGCACGC
CGAGCTTTTCAGGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT166691 WP_001711191.1 NZ_CP058356:29907-30176 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT166691 NZ_CP074393:c597956-597681 [Agrobacterium salinitolerans]
ATGACCGCAAATGCTTATGTGAGAGCGCGCATCGATCAGGCGCTCAAGGATGACGCGACTGCCGTGCTCGACAGTCTGGG
GCTGACTGTTTCCGATGTCATGCGAATGATGCTGACGCGCATTGCCCGAGAAAAAGCACTGCCGATAGAACTGACGCAAC
CGAACGCTGAAACGCTCGCCGCGATTGAAGAAGCGCGCGCCATCGCGGCTGCCAGGCGCAATCGTTTCGGCACGGCGGAA
GCCCTGTTTGACGCCCTTGATGGTAACAAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2