Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4151886..4152383 Replicon chromosome
Accession NZ_CP058355
Organism Escherichia coli strain AMSCJX03

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HXS77_RS19950 Protein ID WP_001173343.1
Coordinates 4151886..4152059 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HXS77_RS19955 Protein ID WP_000398619.1
Coordinates 4152087..4152383 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS77_RS19940 4148556..4150013 + 1458 WP_178887828.1 dipeptide/tripeptide permease DtpC -
HXS77_RS19945 4150250..4151767 + 1518 WP_001295074.1 lysine--tRNA ligase -
HXS77_RS19950 4151886..4152059 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HXS77_RS19955 4152087..4152383 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HXS77_RS19960 4152610..4152882 - 273 WP_000405647.1 N-acetyltransferase -
HXS77_RS19965 4152894..4153124 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HXS77_RS19970 4153305..4154936 + 1632 WP_001565376.1 sensor histidine kinase -
HXS77_RS19975 4154933..4155652 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166690 WP_001173343.1 NZ_CP058355:c4152059-4151886 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166690 NZ_CP074393:c348823-348554 [Agrobacterium salinitolerans]
GTGATCTGGACAATTGAATATCACACGCTTGTCCAGAAAGAGATGCGCAAGATAAATCCCGAAGTGCGCCGGCGTATCCG
CAGCTTTCTCCATGAAAGGCCGGCGGCGCTGGACGACCCGCGCCAAACCGGCGCGGCCCTACAGGGTTCCGAACTCGGAA
ACTACTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCACAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT166690 WP_000398619.1 NZ_CP058355:c4152383-4152087 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166690 NZ_CP074393:c349034-348807 [Agrobacterium salinitolerans]
ATGAGCAAACAGACGGCCATCCGCCTGCCTGATGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGCACGGGCCGCAC
ATCCGCTTACTATATCCGCGAGGCGATCGAGAAACATATCGAGGATATGGAAGACCTTTATCTTGCAGAAGAGGCGACGC
GCCGCATTCAGAACGGTGAATCGAAGGTAATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References