Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3348754..3349372 Replicon chromosome
Accession NZ_CP058355
Organism Escherichia coli strain AMSCJX03

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HXS77_RS16020 Protein ID WP_001291435.1
Coordinates 3349154..3349372 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HXS77_RS16015 Protein ID WP_000344800.1
Coordinates 3348754..3349128 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS77_RS16005 3343843..3345036 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HXS77_RS16010 3345059..3348208 + 3150 WP_178887695.1 efflux RND transporter permease AcrB -
HXS77_RS16015 3348754..3349128 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HXS77_RS16020 3349154..3349372 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HXS77_RS16025 3349544..3350095 + 552 WP_000102564.1 maltose O-acetyltransferase -
HXS77_RS16030 3350211..3350681 + 471 WP_137460524.1 YlaC family protein -
HXS77_RS16035 3350845..3352395 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HXS77_RS16040 3352437..3352790 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HXS77_RS16050 3353169..3353480 + 312 WP_162001342.1 MGMT family protein -
HXS77_RS16055 3353511..3354083 - 573 WP_178887697.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166687 WP_001291435.1 NZ_CP058355:3349154-3349372 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166687 NZ_CP074391:523861-524211 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166687 WP_000344800.1 NZ_CP058355:3348754-3349128 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166687 NZ_CP074391:523574-523855 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References