Detailed information of TA system TA166686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2428420..2429058
• Replicon: chromosome
• Accession: NZ_CP058355
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HXS77_RS11610
• Protein ID: WP_000813794.1
• Coordinates: 2428882..2429058 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HXS77_RS11605
• Protein ID: WP_001270286.1
• Coordinates: 2428420..2428836 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS11585	2423572..2424513	-	942	WP_001251313.1	ABC transporter permease	-
HXS77_RS11590	2424514..2425527	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HXS77_RS11595	2425545..2426690	-	1146	WP_000047418.1	ABC transporter substrate-binding protein	-
HXS77_RS11600	2426935..2428341	-	1407	Protein_2271	PLP-dependent aminotransferase family protein	-
HXS77_RS11605	2428420..2428836	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HXS77_RS11610	2428882..2429058	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HXS77_RS11615	2429280..2429510	+	231	WP_000494244.1	YncJ family protein	-
HXS77_RS11620	2429602..2431563	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HXS77_RS11625	2431636..2432172	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
HXS77_RS11630	2432225..2433439	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T166686 WP_000813794.1 NZ_CP058355:c2429058-2428882 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T166686 NZ_CP074379:2876110-2876412 [Lacticaseibacillus zeae]
ATGTATAGCTTAACGCCTACGCCAACCTTTAAGCGCGACGTTAAACGGCTAGCTAAGAAACATTGGCCGTTAGATGATCT
CAAAACTGCCATTAATCTTCTTGTTACGGGAACAAACGCAGAAACATTGCGTTCAAGATACGCAGATCACGCATTATCTT
CAAGTAGTGATTGGAGAGGATATAGAGAGTTACATGTAAACGGGCCCAAGGGCGACTGGCTGCTCATCTATAAAGTCGAA
CAGCAAAATCTTTTGCTAACCCTAGTCAGGACTGGATCGCACAAGGACCTTTTGGGGAGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT166686 WP_001270286.1 NZ_CP058355:c2428836-2428420 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT166686 NZ_CP074379:2875848-2876117 [Lacticaseibacillus zeae]
ATGGCGACTACAAAAAAGGAAACTCGATTAAACATTCGCATTGATCCCGAATTAAAAGAAGCCGCGCAAGATGTTGCAAG
CGATATGGGAATTGATTTGTCTGCTGCTGTCAACATGTTCATTGTCAAAATGGTCAAAGATCACGCATTACCATTTACAC
CAACCAGTTTACCAGTCGAAACTCTGCAAGCACTTAAGGAAGCAAAGCACTCCGACAAACTAAAGAAGTATCGCACCCCC
GATGACATGTGGGAGGACTTAGATGTATAG

Structures

Toxin

• PDB: 6HPB