Detailed information of TA system TA166685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2344775..2346363
• Replicon: chromosome
• Accession: NZ_CP058355
• Organism: Escherichia coli strain AMSCJX03

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HXS77_RS11260
• Protein ID: WP_001125439.1
• Coordinates: 2345041..2346363 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HXS77_RS11255
• Protein ID: WP_001301023.1
• Coordinates: 2344775..2345041 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS77_RS11245	2340202..2340899	-	698	Protein_2200	IS1 family transposase	-
HXS77_RS11250	2340941..2344552	+	3612	Protein_2201	autotransporter barrel domain-containing lipoprotein	-
HXS77_RS11255	2344775..2345041	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HXS77_RS11260	2345041..2346363	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HXS77_RS11265	2347066..2347763	-	698	Protein_2204	IS1 family transposase	-
HXS77_RS11270	2347818..2350019	+	2202	Protein_2205	acid resistance putative oxidoreductase YdeP	-
HXS77_RS11275	2350267..2350464	+	198	WP_000543394.1	two-component system connector SafA	-
HXS77_RS11280	2350538..2351299	+	762	WP_000060500.1	acid stress response transcriptional regulator YdeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2347066..2347569	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T166685 WP_001125439.1 NZ_CP058355:2345041-2346363 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T166685 NZ_CP074379:c2608094-2607711 [Lacticaseibacillus zeae]
ATGGATGTAACCGTAAAACGCGGAGATGTATTCTTCGCCGATTTATCGCCGGTTGTCGGATCCGAACAGGGTGGCAATCG
ACCTGTACTTATTATTCAAAACAACGTTGGTAACCACTACAGCCCTACGGTTATTGTGGCGGCCATTACCTCCAAGATTC
AGAAACCCAAGATGCCCACTCATGTTGGCTTACGGGCAAAACAAGATGGGGTTGAACGTAACTCGGTGATTTTGCTAGAA
CAGATTCGCACCATCGATAAGCAGCGATTACAGGCGCGGGTGACAGCTTTATCAAGCGCAAAAATGGCGGCCGTTGATCG
TGCGTTGGCAATTTCAGTAGGGCTGGTTAGTTTGCCCAAACCAAAAACATACAATAAAAATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT166685 WP_001301023.1 NZ_CP058355:2344775-2345041 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT166685 NZ_CP074379:c2608362-2608114 [Lacticaseibacillus zeae]
ATGAAGAAGCAAACGTCAGTAACGGTAACGTTTGATGCTGCTATTAAGGCGCGGTTAGATGCCTATTGTGAGTTGAATGA
CACGGATACTCAAGATGTGATCACGACAGCAGTGGCCAAATTTTTGCAGTCACAAGCACCCGATATCAACCAGCTGATTT
CCGGCTACGTTGCCATGGGACAGATCAATACAGAGATCAGTCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU