Detailed information of TA system TA166668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2427547..2428118
• Replicon: chromosome
• Accession: NZ_CP058354
• Organism: Bradyrhizobium japonicum strain 5038

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A845IZB5
• Locus tag: HXT67_RS10885
• Protein ID: WP_014497460.1
• Coordinates: 2427547..2427900 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HXT67_RS10890
• Protein ID: WP_080583987.1
• Coordinates: 2427897..2428118 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXT67_RS10860	2423764..2424000	+	237	WP_014497465.1	hypothetical protein	-
HXT67_RS10865	2424099..2424344	+	246	WP_014497464.1	hypothetical protein	-
HXT67_RS10870	2424459..2425622	+	1164	WP_014497463.1	winged helix-turn-helix domain-containing protein	-
HXT67_RS10875	2425641..2426069	-	429	WP_014497462.1	DUF2809 domain-containing protein	-
HXT67_RS10880	2426150..2427481	+	1332	WP_014497461.1	MFS transporter	-
HXT67_RS10885	2427547..2427900	-	354	WP_014497460.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HXT67_RS10890	2427897..2428118	-	222	WP_080583987.1	antitoxin MazE family protein	Antitoxin
HXT67_RS10895	2428295..2429401	+	1107	WP_028170414.1	efflux RND transporter periplasmic adaptor subunit	-
HXT67_RS10900	2429635..2432772	+	3138	WP_014497458.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (12-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12687.49 Da
• Isoelectric Point: 6.3275

>T166668 WP_014497460.1 NZ_CP058354:c2427900-2427547 [Bradyrhizobium japonicum]
MKRGEIWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDNNGLRVPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAVLVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T166668 NZ_CP074366:3821190-3821468 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8248.25 Da
• Isoelectric Point: 10.4527

>AT166668 WP_080583987.1 NZ_CP058354:c2428118-2427897 [Bradyrhizobium japonicum]
MSPSKPKPSRLKVQAHRDRLRAQGLRPIQIWVPDIRSPSFRSEAHRQSLAVATSVHASEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT166668 NZ_CP074366:3820927-3821187 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A845IZB5