Detailed information of TA system TA166651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 163044..163834
• Replicon: plasmid pPRADMK78_01
• Accession: NZ_CP058351
• Organism: Rhizobium sp. ADMK78

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: FE840_RS18170
• Protein ID: WP_138287434.1
• Coordinates: 163337..163834 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: FE840_RS18165
• Protein ID: WP_138287435.1
• Coordinates: 163044..163340 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE840_RS18135	158895..159653	+	759	WP_171033715.1	ATP-binding cassette domain-containing protein	-
FE840_RS18140	159955..160491	-	537	WP_138287439.1	AhpC/TSA family protein	-
FE840_RS18145	160589..161308	+	720	WP_138287438.1	helix-turn-helix transcriptional regulator	-
FE840_RS18150	161344..161667	-	324	WP_138287437.1	DNA-binding transcriptional regulator	-
FE840_RS18155	161657..162016	-	360	WP_138287436.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FE840_RS18160	162193..162906	+	714	Protein_144	IS6 family transposase	-
FE840_RS18165	163044..163340	+	297	WP_138287435.1	DUF1778 domain-containing protein	Antitoxin
FE840_RS18170	163337..163834	+	498	WP_138287434.1	GNAT family N-acetyltransferase	Toxin
FE840_RS18175	163989..164450	-	462	WP_138287433.1	GNAT family N-acetyltransferase	-
FE840_RS18180	164585..165283	+	699	WP_138287432.1	CerR family C-terminal domain-containing protein	-
FE840_RS18185	165425..166015	-	591	WP_138287431.1	DUF1349 domain-containing protein	-
FE840_RS18190	166138..166209	+	72	Protein_150	IS6 family transposase	-
FE840_RS18195	166841..167086	+	246	WP_138287430.1	hypothetical protein	-
FE840_RS18200	167326..167745	-	420	WP_171033705.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ddhA	1..708533	708533
	-	flank	IS/Tn	-	-	162442..162906	464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-97)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17878.41 Da
• Isoelectric Point: 6.8723

>T166651 WP_138287434.1 NZ_CP058351:163337-163834 [Rhizobium sp. ADMK78]
VTLSAPAPLADHHELAEFNSGVIELDDWLRRRARSNQTSGASRTFVVCEESRVIAYYALASGAVKQPEAPGRFRRNMPDP
IPVAVLGRLAIDQSYQGRGIGRALVRDAGLRLLNAAEVLGIRGMLVHAISDDARAFYEAVGFLSSPSDPMMLMVGLHDLN
NALNP

Nucleotide

• Length: 498 bp

>T166651 NZ_CP074366:295188-295715 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11077.53 Da
• Isoelectric Point: 5.1864

>AT166651 WP_138287435.1 NZ_CP058351:163044-163340 [Rhizobium sp. ADMK78]
MPTSHTSKSSDASVPLNMRIKPASRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTIFTVDPEIYAEYLARLDAPTQ
PNERLARTMSTKAPWDEA

Nucleotide

• Length: 297 bp

>AT166651 NZ_CP074366:294915-295181 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA