Detailed information of TA system TA166640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3974927..3975561
• Replicon: chromosome
• Accession: NZ_CP058349
• Organism: Pseudomonas sp. ABC1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HW090_RS17555
• Protein ID: WP_179114731.1
• Coordinates: 3975157..3975561 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HW090_RS17550
• Protein ID: WP_179114730.1
• Coordinates: 3974927..3975157 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW090_RS17520	3970524..3971093	-	570	WP_179114724.1	DUF4339 domain-containing protein	-
HW090_RS17525	3971274..3971621	+	348	WP_179114725.1	helix-turn-helix domain-containing protein	-
HW090_RS17530	3972009..3972197	-	189	WP_179114726.1	hypothetical protein	-
HW090_RS17535	3972214..3972711	-	498	WP_179114727.1	DNA repair protein RadC	-
HW090_RS17540	3972767..3973681	-	915	WP_179114728.1	hypothetical protein	-
HW090_RS17545	3973762..3974766	-	1005	WP_179114729.1	YqaJ viral recombinase family protein	-
HW090_RS17550	3974927..3975157	+	231	WP_179114730.1	antitoxin	Antitoxin
HW090_RS17555	3975157..3975561	+	405	WP_179114731.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HW090_RS17560	3975635..3976603	-	969	WP_179114732.1	DUF932 domain-containing protein	-
HW090_RS17565	3976721..3976987	+	267	WP_179114733.1	hypothetical protein	-
HW090_RS17570	3977138..3977844	-	707	Protein_3442	transposase	-
HW090_RS17575	3977916..3978104	-	189	WP_179114959.1	formate dehydrogenase subunit delta	-
HW090_RS17580	3978247..3978566	-	320	Protein_3444	hypothetical protein	-
HW090_RS17585	3978848..3979723	-	876	WP_179114734.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3952313..3993524	41211
	-	flank	IS/Tn	-	-	3977536..3977844	308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15097.26 Da
• Isoelectric Point: 7.2782

>T166640 WP_179114731.1 NZ_CP058349:3975157-3975561 [Pseudomonas sp. ABC1]
MLKFMLDTNICIFTIKNRPEQVRETFKRHSGQLSISTITLMELIYGAEKSSNPERNLADVEGFAARLDVLPYDSQAATHS
GQLRSELARVGKPIGPYDQMIAGHARSQGLILVTNNLREFERVPGLRVEDWVNP

Nucleotide

• Length: 405 bp

>T166640 NZ_CP074364:3395972-3396229 [Xanthomonas translucens pv. cerealis]
GTGACGGTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAACGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAAGGCGTCGGCAAGCCAGAACCGCTGCGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCTGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8450.38 Da
• Isoelectric Point: 4.2683

>AT166640 WP_179114730.1 NZ_CP058349:3974927-3975157 [Pseudomonas sp. ABC1]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRTRIITPAGEAWDSWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT166640 NZ_CP074364:3395724-3395975 [Xanthomonas translucens pv. cerealis]
ATGGACACCATCACCTATAGCGCTGCACGCGCTGCCCTTGCGGACACCATGGAGCGCGTGGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCTCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA