Detailed information of TA system TA166636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 566800..567581
• Replicon: chromosome
• Accession: NZ_CP058349
• Organism: Pseudomonas sp. ABC1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HW090_RS02330
• Protein ID: WP_179111965.1
• Coordinates: 567090..567581 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HW090_RS02325
• Protein ID: WP_134587219.1
• Coordinates: 566800..567090 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW090_RS02310	564454..565239	+	786	WP_179111963.1	thioredoxin domain-containing protein	-
HW090_RS02315	565406..565900	+	495	WP_179114794.1	DNA repair protein RadC	-
HW090_RS02320	566138..566746	+	609	WP_179111964.1	hypothetical protein	-
HW090_RS02325	566800..567090	+	291	WP_134587219.1	DUF1778 domain-containing protein	Antitoxin
HW090_RS02330	567090..567581	+	492	WP_179111965.1	GNAT family N-acetyltransferase	Toxin
HW090_RS02335	567689..568105	+	417	WP_179111966.1	TIGR03757 family integrating conjugative element protein	-
HW090_RS02340	568102..569052	+	951	WP_179111967.1	TIGR03756 family integrating conjugative element protein	-
HW090_RS02345	569063..570457	+	1395	WP_179111968.1	integrating conjugative element protein	-
HW090_RS02350	570454..570822	+	369	WP_179111969.1	hypothetical protein	-
HW090_RS02355	570836..572353	+	1518	WP_179111970.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	514362..627016	112654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-96)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17563.20 Da
• Isoelectric Point: 9.6232

>T166636 WP_179111965.1 NZ_CP058349:567090-567581 [Pseudomonas sp. ABC1]
MPLSAPQPLAAHHDVQSFSSGVDSLDQWLKRRALKNQTTGASRTFVVCEDKRVLAYYALASSAVISAEASGRFRRNMPDP
IPVVVLGRLAVDQSLQGQGFGRALVQDAGRRVIQAADAIGIRGMLVHALSPEAKAFYERIGFEPSALDPMTLMTTLADLK
ASL

Nucleotide

• Length: 492 bp

>T166636 NZ_CP074364:278278-278430 [Xanthomonas translucens pv. cerealis]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTCTGTGCAGGCATGCTGACTGGCTGCAACACCATGGCCGGTGC
CGGCAAGGATATGCAGAAGGCCGGCGATAAGGTGGAAAACAAGGCCGACGACTGCAAAGACGGCAAGTGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10873.52 Da
• Isoelectric Point: 10.2614

>AT166636 WP_134587219.1 NZ_CP058349:566800-567090 [Pseudomonas sp. ABC1]
MSTTLNITPGKRETLNLRIKPEERSLIDRAAKVRGKNRTDFVLEAARAAAEEALLDQSIIAVDQEAYAAFLARLDMPPQP
NERLRKTMRTPAPWEK

Nucleotide

• Length: 291 bp

>AT166636 NZ_CP074364:278529-278663 [Xanthomonas translucens pv. cerealis]
ATGAAGCGTACTTTTGCGTGGATGCTGCTGGCGATGTTTTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA