Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4306048..4306545 Replicon chromosome
Accession NZ_CP058342
Organism Escherichia coli strain W60

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HXP40_RS20510 Protein ID WP_001173343.1
Coordinates 4306048..4306221 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HXP40_RS20515 Protein ID WP_000398619.1
Coordinates 4306249..4306545 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXP40_RS20500 4302718..4304175 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HXP40_RS20505 4304412..4305929 + 1518 WP_001295074.1 lysine--tRNA ligase -
HXP40_RS20510 4306048..4306221 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HXP40_RS20515 4306249..4306545 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HXP40_RS20520 4306772..4307044 - 273 WP_000405651.1 N-acetyltransferase -
HXP40_RS20525 4307056..4307286 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HXP40_RS20530 4307467..4309098 + 1632 WP_001216477.1 sensor histidine kinase -
HXP40_RS20535 4309095..4309814 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166618 WP_001173343.1 NZ_CP058342:c4306221-4306048 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166618 NZ_CP074359:2613952-2614059 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT166618 WP_000398619.1 NZ_CP058342:c4306545-4306249 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166618 NZ_CP074359:c2613902-2613839 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References