Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3535164..3535782 Replicon chromosome
Accession NZ_CP058342
Organism Escherichia coli strain W60

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HXP40_RS16795 Protein ID WP_001291435.1
Coordinates 3535564..3535782 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HXP40_RS16790 Protein ID WP_000344800.1
Coordinates 3535164..3535538 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXP40_RS16780 3530253..3531446 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HXP40_RS16785 3531469..3534618 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HXP40_RS16790 3535164..3535538 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HXP40_RS16795 3535564..3535782 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HXP40_RS16800 3535954..3536505 + 552 WP_000102564.1 maltose O-acetyltransferase -
HXP40_RS16805 3536621..3537091 + 471 WP_000136192.1 YlaC family protein -
HXP40_RS16810 3537255..3538805 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HXP40_RS16815 3538847..3539200 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HXP40_RS16825 3539579..3539890 + 312 WP_000409911.1 MGMT family protein -
HXP40_RS16830 3539921..3540493 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166613 WP_001291435.1 NZ_CP058342:3535564-3535782 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166613 NZ_CP074359:1974579-1974682 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166613 WP_000344800.1 NZ_CP058342:3535164-3535538 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166613 NZ_CP074359:c1974720-1974449 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References