Detailed information of TA system TA166598

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 797058..797890
• Replicon: chromosome
• Accession: NZ_CP058342
• Organism: Escherichia coli strain W60

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: HXP40_RS03825
• Protein ID: WP_000854765.1
• Coordinates: 797058..797432 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HXP40_RS03830
• Protein ID: WP_001295723.1
• Coordinates: 797522..797890 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP40_RS03790	792136..792735	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
HXP40_RS03795	792738..793715	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
HXP40_RS03800	793712..794890	+	1179	WP_000094991.1	type II secretion system protein GspL	-
HXP40_RS03805	794892..795428	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
HXP40_RS03810	795709..796551	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
HXP40_RS03815	796636..796830	-	195	WP_024181941.1	DUF957 domain-containing protein	-
HXP40_RS03820	796855..797058	-	204	WP_001398797.1	hypothetical protein	-
HXP40_RS03825	797058..797432	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
HXP40_RS03830	797522..797890	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HXP40_RS03835	798053..798274	-	222	WP_000692341.1	DUF987 domain-containing protein	-
HXP40_RS03840	798337..798813	-	477	WP_001186774.1	RadC family protein	-
HXP40_RS03845	798829..799308	-	480	WP_057109541.1	antirestriction protein	-
HXP40_RS03850	799574..800392	-	819	WP_001175165.1	DUF945 domain-containing protein	-
HXP40_RS03855	800482..800715	-	234	WP_001278293.1	DUF905 family protein	-
HXP40_RS03860	800721..801398	-	678	WP_001097302.1	hypothetical protein	-
HXP40_RS03865	801546..802226	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T166598 WP_000854765.1 NZ_CP058342:c797432-797058 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T166598 NZ_CP074356:c4163799-4163512 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT166598 WP_001295723.1 NZ_CP058342:c797890-797522 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT166598 NZ_CP074356:c4163525-4163223 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52