Detailed information of TA system TA166594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 9716..10634
• Replicon: plasmid unnamed
• Accession: NZ_CP058341
• Organism: Campylobacter coli strain 20A420

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: HXP32_RS08580
• Protein ID: WP_065980145.1
• Coordinates: 9951..10634 (+)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: A0A0Q2TTN4
• Locus tag: HXP32_RS08575
• Protein ID: WP_002804094.1
• Coordinates: 9716..9958 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP32_RS08555	4717..4857	+	141	WP_002779802.1	hypothetical protein	-
HXP32_RS08560	4862..7075	+	2214	WP_002795783.1	DUF1738 domain-containing protein	-
HXP32_RS08565	7072..8895	+	1824	WP_065980146.1	type IV secretory system conjugative DNA transfer family protein	-
HXP32_RS08570	8906..9625	+	720	WP_002784845.1	conjugal transfer protein TraL	-
HXP32_RS08575	9716..9958	+	243	WP_002804094.1	hypothetical protein	Antitoxin
HXP32_RS08580	9951..10634	+	684	WP_065980145.1	Fic family protein	Toxin
HXP32_RS08585	10639..11106	+	468	WP_057039919.1	signal peptidase I	-
HXP32_RS08590	11171..11566	+	396	WP_004294083.1	conjugal transfer protein TraM	-
HXP32_RS08595	11658..12344	+	687	WP_002791558.1	AAA family ATPase	-
HXP32_RS08600	12347..12571	+	225	WP_002790621.1	hypothetical protein	-
HXP32_RS08605	12812..14968	-	2157	WP_065980144.1	relaxase/mobilization nuclease domain-containing protein	-
HXP32_RS08610	14931..15314	-	384	WP_065980143.1	DNA transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26486	26486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 25799.74 Da
• Isoelectric Point: 10.0983

>T166594 WP_065980145.1 NZ_CP058341:9951-10634 [Campylobacter coli]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKKERIITLQAAKKLELNPIKGNFDYQHLKDIHKALFKDVYTWA
GKDRMEVGLHGNFGKYAPNGTITNFVPGKDLNVTAQQIFTWLKEDNYLKNSKDLNDFAKNLAEFARNLNALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLIPKDKTIMASVEASQLKLGKLEAIIKTNLKSFRQNLDLEQNKGISL

Nucleotide

• Length: 684 bp

>T166594 NZ_CP074356:3133327-3133713 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9452.68 Da
• Isoelectric Point: 4.3535

>AT166594 WP_002804094.1 NZ_CP058341:9716-9958 [Campylobacter coli]
MQKQYPEVHSLEESLAILKKYKDDLTKEQYEQNKSIICGFAIENMFANEEDIINLIKVDKQEKTPDEIIAEYKKEWGVND

Nucleotide

• Length: 243 bp

>AT166594 NZ_CP074356:3133703-3134032 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0Q2TTN4