Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3014322..3014936 Replicon chromosome
Accession NZ_CP058332
Organism Pseudomonas aeruginosa strain B18

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag B18_RS13850 Protein ID WP_071534354.1
Coordinates 3014322..3014504 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag B18_RS13855 Protein ID WP_012077229.1
Coordinates 3014532..3014936 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B18_RS13825 3009963..3010358 + 396 WP_003094374.1 hypothetical protein -
B18_RS13830 3010351..3011727 + 1377 WP_003098863.1 class II fumarate hydratase -
B18_RS13835 3011755..3012204 + 450 WP_003094369.1 hypothetical protein -
B18_RS13840 3012217..3012828 + 612 WP_003098862.1 superoxide dismutase -
B18_RS13845 3012847..3013779 + 933 WP_003120918.1 ZIP family metal transporter -
B18_RS13850 3014322..3014504 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
B18_RS13855 3014532..3014936 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B18_RS13860 3014979..3015950 - 972 WP_012077228.1 hypothetical protein -
B18_RS13865 3015935..3016633 - 699 WP_033896049.1 hypothetical protein -
B18_RS13870 3016633..3017172 - 540 WP_012074127.1 hypothetical protein -
B18_RS13875 3017169..3018089 - 921 WP_012074128.1 hypothetical protein -
B18_RS13880 3018086..3018796 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3014322..3051816 37494


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T166582 WP_071534354.1 NZ_CP058332:3014322-3014504 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T166582 NZ_CP074356:c597705-597241 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT166582 WP_012077229.1 NZ_CP058332:3014532-3014936 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT166582 NZ_CP074356:c598040-597705 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References